Anti-LBR Polyclonal Antibody for WB, IF/ICC, ELISA - Q14739

Core Product Specifications and Parameters

Biological Background: LBR Function and Localization

• LBR (lamin B receptor) is a dual-function protein possessing both sterol reductase activity and nuclear envelope anchoring capabilities.
• Catalyzes the reduction of the C14-unsaturated bond of lanosterol, a key step in the cholesterol biosynthesis pathway. Related references: PMID:12618959, PMID:16784888, PMID:21327084
• Plays a critical role in myeloid cell cholesterol biosynthesis, essential for cell growth, functional maturation, and possibly NADPH oxidase activation via membrane lipid raft formation.
• Anchors the lamina and heterochromatin to the inner nuclear membrane, contributing to nuclear architecture and chromatin organization. Related references: PMID:10828963
• Localizes to the nucleus inner membrane, endoplasmic reticulum membrane, cytoplasm, and nucleus, reflecting its multi-compartmental functions.
• Expressed in a wide range of tissues including bone marrow, liver, heart, adrenal gland, lung, placenta, uterus, and in osteoclasts and osteoblast-like cells. Related references: PMID:16784888, PMID:21327084
• Post-translational modifications such as acetylation and phosphorylation regulate its function, and mutations are linked to Pelger-Huet anomaly and dwarfism.

Experimental Guidance and Technical Tips

• For Western blot, LBR migrates near its predicted molecular weight of ~71 kDa. Validate band specificity using appropriate positive and negative controls, as post-translational modifications may cause slight shifts.
• In immunofluorescence/ICC, LBR exhibits a characteristic nuclear rim and endoplasmic reticulum staining pattern. Optimize fixation and permeabilization conditions to preserve epitope accessibility.
• ELISA applications may require careful selection of coating antigen and detection conditions; recombinant LBR protein or cell lysates can be used.
• Cross-reactivity against mouse and rat is confirmed; consider validating in other species if required, based on sequence homology of the immunogen region.
• The immunogen corresponds to the N-terminal region (amino acids 1-211), which may detect multiple potential isoforms or cleavage products; verify in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering comprehensive support from concept to clinic. Our LBR polyclonal antibody enables robust detection in WB, IF/ICC, and ELISA, serving research in cholesterol metabolism and nuclear envelope biology. Beyond this antibody, we provide validated antibody pairs, bulk ancillary reagents, and customized raw-material sourcing to meet your diagnostic development needs.