Anti-LATS2 Rabbit Polyclonal Antibody for WB, ELISA - Q9NRM7

Core Product Specifications and Parameters

Biological Background: LATS2 Function and Localization

• LATS2 (serine/threonine-protein kinase LATS2) is also known as kinase phosphorylated during mitosis protein (KPM), large tumor suppressor homolog 2, and warts-like kinase. It is a core component of the Hippo signaling pathway.
• LATS2 negatively regulates YAP1 by phosphorylating it and inhibiting its nuclear translocation. This action restricts proliferation and promotes apoptosis, acting as a key tumor suppressor. Related references: PMID:18158288 PMID:26437443 PMID:26598551 PMID:34404733
• Within the Hippo kinase cascade, MST1/2 (STK4/3) in complex with SAV1 phosphorylates and activates LATS2 in complex with MOB1. Activated LATS2 then phosphorylates YAP1 and WWTR1/TAZ. Related references: PMID:26437443 PMID:26598551 PMID:34404733
• LATS2 plays a critical role in centrosome duplication, maintenance of mitotic fidelity, and genomic stability. It also negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Related references: PMID:10871863 PMID:12853976
• Subcellularly, LATS2 localizes to the cytoplasm, centrosome, spindle poles, and nucleus.
• Tissue specificity: LATS2 is expressed at high levels in heart and skeletal muscle, with lower expression in other tissues.
• Additional functions include phosphorylation of SNAI1 (promoting EMT and tumor invasion) and activation of the NLRP3 inflammasome via phosphorylation of NLRP3. Related references: PMID:21952048 PMID:39173637

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against either a recombinant protein or a synthetic peptide from the N-terminal region (amino acids 70-145) of human LATS2, providing multi-epitope recognition.
• For Western blotting, LATS2 runs at approximately 120 kDa. Choose an appropriate gel percentage (e.g., 8% SDS-PAGE) and optimize transfer conditions for high-molecular-weight proteins.
• In ELISA, consider both direct and indirect formats depending on the detection system. Titrate the antibody to determine optimal working concentration.
• Cross-reactivity with mouse and rat LATS2 is expected based on supplier data. Validate in the relevant species and tissue, noting that expression levels vary (higher in heart and skeletal muscle).
• Non-committal suggestions: verify specificity by testing in LATS2 knockout or knockdown samples if available, and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research labs with one-stop access to IVD raw materials, from concept to clinic. This anti-LATS2 polyclonal antibody supports studies in tumor biology, Hippo signaling, and cell cycle regulation. Explore our portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your assay development and research.