Anti-LATS1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O95835

Core Product Specifications and Parameters

Biological Background: LATS1 Function and Localization

• LATS1 (Large tumor suppressor homolog 1), also known as WARTS, is a serine/threonine-protein kinase encoded by the LATS1 gene and belongs to the Hippo signaling pathway.
• Phosphorylation of YAP1 by LATS1 inhibits its nuclear translocation, suppressing cell proliferation and promoting apoptosis, thereby controlling organ size and tumor suppression. Related references: PMID:10518011, PMID:10831611, PMID:18158288
• The core kinase cascade involves STK3/MST2 and STK4/MST1 in complex with SAV1, which phosphorylate LATS1/2 in complex with MOB1, leading to phosphorylation and inactivation of YAP1 and WWTR1/TAZ. Related references: PMID:18158288, PMID:26437443, PMID:28068668
• Acts as a tumor suppressor maintaining ploidy through regulation of mitotic progression and the G1 tetraploidy checkpoint. Related references: PMID:15122335, PMID:19927127
• Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Related references: PMID:9988268
• Affects cytokinesis by regulating actin polymerization via negative modulation of LIMK1. Related references: PMID:15220930
• Plays a role in mammary gland epithelial cell differentiation through the Hippo pathway and by promoting degradation of ESR1. Related references: PMID:28068668
• Activates the NLRP3 inflammasome by mediating phosphorylation of NLRP3 at Ser-265, enabling NLRP3 activation by NEK7. Related references: PMID:39173637
• Subcellular localization includes centrosome, spindle, midbody, and spindle pole body, consistent with its roles in mitosis and cytokinesis.
• Expressed ubiquitously in adult tissues except lung and kidney.

Experimental Guidance and Technical Tips

• For Western blot (WB), note the predicted molecular weight of LATS1 is ~127 kDa. Consider using a low percent SDS-PAGE gel (e.g., 6–8%) and appropriate transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P), antigen retrieval may be required; validate the optimal method in your tissue samples. Positive control tissues should be selected based on known expression (e.g., tissues other than lung or kidney).
• For ELISA, the immunogen (synthetic peptide corresponding to amino acids 100–200) may serve as a positive control. Include appropriate negative controls to assess specificity.
• As a polyclonal antibody, batch-to-batch consistency should be confirmed for critical assays. Consider validating reactivity in the relevant species (human, mouse, rat) and sample types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For researchers and diagnostic developers focused on the Hippo pathway and tumor suppressors like LATS1, CamelBio can efficiently supply this key reagent and assist with rare target sourcing. From concept to clinic, rely on CamelBio as your integrated partner for all raw-material needs.