Anti-LARS Polyclonal Antibody for WB, IF/ICC, ELISA - Q9P2J5

Core Product Specifications and Parameters

Biological Background: LARS Function and Localization

• LARS (Leucyl-tRNA synthetase, cytoplasmic) catalyzes the specific attachment of leucine to its cognate tRNA(Leu), a critical step in cytoplasmic protein synthesis. Related references: PMID:25051973, PMID:32232361
• It employs a two-step mechanism: leucine activation by ATP to form leucyl-AMP, followed by transfer to tRNA. Editing functions hydrolyze misactivated adenylates and mischarged tRNAs to ensure translational fidelity. Related references: PMID:25051973
• The protein localizes to the cytoplasm, consistent with its role in cytosolic translation.
• LARS is subject to post-translational modifications such as acetylation and phosphorylation, potentially modulating its activity.
• Alternative splicing of LARS1 transcripts may generate isoforms with distinct regulatory functions.
• Mutations in LARS1 are linked to human diseases, including infantile liver failure syndrome type 1 (ILFS1) (by disease variant annotation).
• The protein contains ATP-binding and nucleotide-binding domains essential for its catalytic activity.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-270) targets the N-terminal region of LARS; consider its suitability for detecting both full-length and N-terminal fragments.
• For Western blot, the predicted molecular weight is 134 kDa; use appropriate gel percentage and transfer conditions for high molecular weight proteins.
• In immunofluorescence/immunocytochemistry, validate antibody specificity with proper controls, noting reported cross-reactivity with mouse.
• For ELISA, include relevant positive and negative controls to ensure signal specificity.
• Pre-adsorption with the immunogen can be used to confirm specificity in appropriate experimental systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with validated antibody reagents and ancillary raw materials. This anti-LARS polyclonal antibody is suitable for developing immunoassays targeting aminoacyl-tRNA synthetase pathways. CamelBio offers a streamlined sourcing experience from concept to clinic.