Anti-LARP7 Rabbit Polyclonal Antibody for WB, ELISA - Q4G0J3

Core Product Specifications and Parameters

Biological Background: LARP7 Function and Localization

• LARP7 (La-related protein 7), also known as hLARP7 or PIP7S, is an RNA-binding protein that specifically binds distinct small nuclear RNAs (snRNAs) to regulate their processing and function. Related references: PMID:18249148 PMID:32017898
• It acts as a core component of the 7SK ribonucleoprotein (RNP) complex, which sequesters the positive transcription elongation factor b (P-TEFb) in an inactive state, negatively regulating RNA polymerase II transcriptional elongation. Related references: PMID:18249148 PMID:18483487
• Upon stimulation, LARP7 remains associated with 7SK RNA while P-TEFb is released, enabling dynamic transcriptional control. Related references: PMID:18281698 PMID:18483487
• LARP7 also facilitates snRNA gene transcription by RNA polymerase II through interaction with the little elongation complex (LEC). Related references: PMID:28254838
• Beyond transcription, LARP7 ensures mRNA splicing fidelity by binding U6 snRNA and promoting its 2'-O-methylation via box C/D RNP complexes; U6 snRNA processing is essential for spermatogenesis. Related references: PMID:32017898
• The protein primarily localizes to the nucleoplasm and undergoes post-translational modifications such as acetylation, phosphorylation, and ubiquitination. Mutations in LARP7 are linked to dwarfism and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 160–380 of human LARP7, covering a region likely involved in RNA binding. When performing WB, a band around 67 kDa is expected; consider using positive controls like HeLa or HEK293T lysates.
• For ELISA, optimization of coating antigen and antibody dilution is recommended. Since this is a polyclonal antibody, lot-to-lot variation may occur; include appropriate negative controls in each experiment.
• The antibody cross-reacts with mouse LARP7, making it suitable for studies in mouse models. Validation in the relevant sample system is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop sourcing for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-LARP7 antibody supports research on transcriptional regulation and mRNA splicing, and we can assist in sourcing rare targets or custom formulations to accelerate your project development.