Anti-LARP5 Polyclonal Antibody for WB, ELISA - Q92615

Core Product Specifications and Parameters

Biological Background: LARP5 Function and Localization

• La-related protein 4B (LARP4B), also known as LARP5 or KIAA0217, is a member of the La-related protein family characterized by La motif and RNA recognition motifs.
• LARP4B functions as a positive regulator of mRNA translation, enhancing protein synthesis from target transcripts.
• The protein contains an N-terminal La motif (LAM) and a conserved RNA-binding domain, suggesting direct interaction with RNA.
• Subcellularly, LARP4B localizes predominantly to the cytoplasm and cytosol, consistent with its role in translational regulation.
• Post-translational modifications include acetylation, methylation, and phosphorylation, indicating potential regulation by cellular signaling pathways.
• Keywords associated with LARP4B include RNA-binding, translation regulation, and phosphoprotein, highlighting its central role in post-transcriptional control.

Experimental Guidance and Technical Tips

• The immunogen encompasses the C-terminal region (aa 519-738), which may contain less conserved sequences; consider using this antibody for detecting full-length LARP4B or its fragments.
• For Western blotting, the predicted molecular weight is 81 kDa; include appropriate molecular weight markers and validate in relevant lysates (human, mouse, rat).
• Cross-reactivity with mouse and rat is indicated, making this antibody suitable for cross-species studies; however, verify specificity in your experimental system.
• For ELISA, optimize coating and detection conditions; the antibody is polyclonal, increasing sensitivity but may require careful blocking.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting research into mRNA translation and gene expression. We offer a comprehensive portfolio of validated antibodies, including polyclonal and monoclonal options, along with ancillary reagents to accelerate your assay development. Our team assists with material selection and supply for rare targets like LARP4B, ensuring seamless integration from concept to clinic.