Anti-LARP4 Polyclonal Antibody for WB, IF/ICC, ELISA - Q71RC2

Core Product Specifications and Parameters

Biological Background: LARP4 Function and Localization

• LARP4 is an RNA-binding protein that specifically recognizes poly-A tails of mRNAs and associates with 40S ribosomal subunits and polysomes, playing a key role in mRNA translation regulation. Related references: PMID:21098120
• It is essential for proper cell morphology and cytoskeletal organization, as evidenced by its requirement for normal actin filament dynamics. Related references: PMID:21834987, PMID:27615744
• Subcellularly, LARP4 localizes predominantly in the cytoplasm, including the cytosol and stress granules, where it participates in mRNA metabolism under stress conditions.
• The protein undergoes various post-translational modifications, including acetylation, methylation, and phosphorylation, which may regulate its activity and interactions.
• LARP4 is a member of the La ribonucleoprotein domain family, which includes other RNA-binding proteins implicated in diverse aspects of RNA metabolism.
• As a reference proteome member, LARP4 has been identified through proteomics and direct protein sequencing, underscoring its expression and functional significance in human cells.
• Alternative splicing generates multiple transcript variants, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (aa 1-120); the resulting polyclonal antibody may recognize full-length LARP4 and possibly truncated isoforms—validate in your target tissue or cell line.
• For Western blotting, the predicted molecular weight is 81 kDa; use appropriate controls (e.g., knockout lysates or blocking peptides) to confirm band specificity in human, mouse, or rat samples.
• In immunofluorescence/ICC, LARP4 distributes in the cytoplasm and stress granules; consider inducing stress granules with sodium arsenite and optimize fixation/permeabilization to detect native localization.
• ELISA applications can be developed using recombinant LARP4 or cell lysates, but careful titration and blocking are advised to minimize background.
• As with any polyclonal antibody, lot-to-lot variability may occur; always validate performance in your experimental system before large-scale studies.
• Reported cross-reactivity with mouse and rat should be independently verified using appropriate species-specific protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a reliable, one-stop channel for sourcing high-quality IVD raw materials. This anti-LARP4 polyclonal antibody supports studies in RNA biology, translation regulation, and cytoskeletal dynamics. Beyond individual antibodies, we offer validated antibody pairs, bulk monoclonal/polyclonal reagents, and custom sourcing for rare targets to streamline your assay development and manufacturing needs.