Anti-LARP1 Rabbit Polyclonal Antibody for WB, ELISA - Q6PKG0

Core Product Specifications and Parameters

Biological Background: LARP1 Function and Localization

• LARP1 (La-related protein 1) is an RNA-binding protein that regulates the translation of specific target mRNAs downstream of the mTORC1 complex in response to growth signals and nutrient availability. Related references: PMID:20430826, PMID:23711370, PMID:24532714
• It interacts with both the 3' poly-A tails and the 5' terminal oligopyrimidine (5'TOP) motifs of mRNAs, repressing translation of 5'TOP-containing transcripts by preventing EIF4G1 binding. Related references: PMID:23711370, PMID:25940091, PMID:26206669, PMID:28379136, PMID:28650797
• Upon mTORC1 activation, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR), relieving translational repression. Related references: PMID:25940091, PMID:28650797
• Under normal nutrient availability, LARP1 primarily associates with the 3' UTR of ribosomal protein mRNAs and stimulates their translation, associating with actively translating ribosomes. Related references: PMID:23711370, PMID:28650797
• LARP1 stabilizes mRNAs containing a 5'TOP motif, a process required to prevent apoptosis. Related references: PMID:20430826, PMID:23711370, PMID:25940091
• It localizes predominantly to the cytoplasm and can be found in cytoplasmic granules.
• Positively regulates the replication of dengue virus (DENV) upon infection.
• Keywords: Acetylation, Alternative splicing, Direct protein sequencing, Isopeptide bond, Methylation, Phosphoprotein, Protein biosynthesis, Repressor, RNA-binding, Translation regulation, Ubl conjugation.

Experimental Guidance and Technical Tips

• For Western blotting, given the large predicted molecular weight (124 kDa), consider using a low-percentage SDS-PAGE gel (e.g., 6–8%) and extended transfer times to ensure efficient detection.
• In ELISA applications, the recombinant immunogen (amino acids 106–205) may serve as a useful positive control for antigen-coating optimization.
• As the antibody is raised against a human-specific immunogen, validate reactivity in the relevant human cell lines or tissue lysates and consider testing cross-reactivity with other species if required.
• Because LARP1 is a phosphoprotein regulated by mTORC1 signaling, ensure that cell culture conditions (serum levels, nutrient status) are controlled to interpret expression and modification patterns accurately.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with an integrated portfolio of validated IVD raw materials, including optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. For LARP1-related projects—particularly those investigating translational control, mTORC1 signaling, or stress granule biology—we can facilitate access to complementary antibody pairs, recombinant proteins, and custom development services. From early discovery to clinical assay development, our technical consultancy and supply chain solutions are designed to accelerate your workflow.