Anti-LAR Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P10586

Core Product Specifications and Parameters

Biological Background: LAR (Receptor-type tyrosine-protein phosphatase F) Function and Localization

• Encodes receptor-type tyrosine-protein phosphatase F (PTPRF), also known as leukocyte common antigen related (LAR), a transmembrane receptor with intrinsic protein tyrosine phosphatase activity.
• Functions as a cell adhesion receptor and regulates signaling by dephosphorylating substrates such as EPHA2, thereby modulating its activity.
• Contains two intracellular PTPase domains: the first domain harbors catalytic activity, while the second domain influences the substrate specificity of the first.
• Localized to the cell membrane, consistent with its role as a receptor and adhesion molecule.
• Possesses extracellular immunoglobulin-like and fibronectin type III domains that mediate cell adhesion and ligand interactions.
• Undergoes post-translational modifications including glycosylation, disulfide bond formation, and phosphorylation, as indicated by UniProt keywords.
• Alternative splicing generates multiple isoforms, potentially affecting receptor function or ligand binding.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein spanning amino acids 1016–1250 of human PTPRF (NP_002831.2), corresponding to an intracellular region; verify reactivity against the specific isoform in your experimental system.
• For Western blot, expect a band at approximately 213 kDa under reducing conditions; use appropriate molecular weight markers and validate in relevant cell lysates expressing PTPRF.
• In immunohistochemistry on paraffin-embedded sections (IHC-P), membrane staining is anticipated; optimize antigen retrieval conditions and include suitable positive and negative controls.
• For ELISA applications, the antibody may be suitable for detecting recombinant or native PTPRF; pairing with a detection antibody and validating in a sandwich format is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers a streamlined sourcing solution for IVD raw materials, from validated antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents. For receptor tyrosine phosphatase research, our network supports targets like PTPRF/LAR, helping bridge the gap from concept to clinical application. Contact our team for customized or rare-target raw-material sourcing.