Anti-LAPTM5 Rabbit Polyclonal Antibody for WB, ELISA - Q13571

Core Product Specifications and Parameters

Biological Background: LAPTM5 Function and Localization

• LAPTM5 (Lysosomal-associated transmembrane protein 5), also known as CLAST6 and Retinoic acid-inducible E3 protein, is a multi-pass transmembrane protein.
• May play a special functional role during embryogenesis and in the maintenance or function of adult hematopoietic cells.
• Localizes predominantly to the lysosomal membrane, where it likely participates in lysosomal transport or signaling.
• Preferentially expressed in adult hematopoietic tissues, with high levels in peripheral blood leukocytes, thymus, spleen, and lung; also detected in placenta, liver, and kidney.
• Annotated as a phosphoprotein, suggesting regulation by phosphorylation.
• Contains transmembrane helices and is classified under transport-related keywords, indicating a potential role in substrate translocation across the lysosomal membrane.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 153 to 262 of human LAPTM5, a region likely exposed on cytoplasmic or luminal loops; consider using reducing conditions for Western blotting to resolve the 30 kDa band.
• For Western blot, optimize primary antibody dilution using relevant positive controls such as hematopoietic cell lines (e.g., Jurkat, Raji) or tissue lysates from spleen or thymus.
• For ELISA applications, test the antibody in an indirect or capture format with the recombinant immunogen or native protein from cell/tissue extracts; coating and detection conditions may require optimization.
• Cross-reactivity is reported for human, mouse, and rat; validate performance in your specific experimental system, as reactivity patterns may vary by tissue or cell type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials. For studies involving hematopoietic biology, lysosomal function, or immunology, we provide validated anti-LAPTM5 polyclonal antibodies, ideal for WB and ELISA workflows. Beyond this product, CamelBio offers optimized antibody pairs, monoclonal antibodies, and bulk ancillary reagents, efficiently bridging rare-target sourcing gaps to accelerate your assay development and production.