Anti-LAPTM4B Rabbit Polyclonal Antibody for WB, ELISA - Q86VI4

Core Product Specifications and Parameters

Biological Background: LAPTM4B Function and Localization

• LAPTM4B, also known as lysosomal-associated transmembrane protein 4-beta (LAPTM4β) and LC27, is encoded by the LAPTM4B gene in humans.
• Required for optimal lysosomal function. Related references: PMID:21224396
• Blocks EGF-stimulated EGFR intraluminal sorting and degradation; regulates PIP5K1C interaction and inhibits HGS ubiquitination. Related references: PMID:25588945
• Recruits the leucine transporter complex SLC3A2-SLC7A5 to lysosomes, promoting essential amino acid entry and activating V-ATPase and mTORC1 signaling. Related references: PMID:25998567
• Functions as a negative regulator of TGFB1 production in regulatory T cells. Related references: PMID:26126825
• Binds ceramide and facilitates its exit from late endosomes, thereby controlling cell death pathways. Related references: PMID:26280656
• Localizes to the endomembrane system, including late endosome, lysosome, multivesicular body, and cell membrane, and is found in cell projections.
• Annotated with keywords including alternative splicing, transmembrane helix, transport, and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant fusion protein corresponding to the cytosolic region of human LAPTM4B (amino acids 218-317), which may provide specificity for detecting the full-length protein.
• For Western blot (WB), consider using a dilution range of 1:500–1:2000 and validate in lysates that endogenously express LAPTM4B, such as lysosome-enriched fractions or cancer cell lines known to upregulate LAPTM4B.
• For ELISA, titration is recommended to determine the optimal coating concentration and detection range; include proper controls for nonspecific binding.
• As the antibody cross-reacts with mouse and rat LAPTM4B, it may be suitable for interspecies studies; however, verify target sequence homology in the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD developers, offering a broad portfolio of antibodies and ancillary reagents. This anti-LAPTM4B rabbit polyclonal antibody suits research in lysosomal biology, mTORC1 signaling, and cancer. Beyond individual antibodies, CamelBio supplies validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and specialized rare-target raw materials—supporting your entire assay development pipeline from concept to clinic.