Anti-LAP3 Rabbit Polyclonal Antibody - P28838

Core Product Specifications and Parameters

Biological Background: LAP3 Function and Localization

• LAP3 encodes cytosol aminopeptidase, a zinc-dependent metallopeptidase that cleaves N-terminal hydrophobic amino acids from peptides. The presence of Zn²⁺ ions is essential for its catalytic activity, while cofactors like Mn²⁺ can modulate substrate specificity.
• It plays a critical role in glutathione metabolism by hydrolyzing Cys-Gly bonds of glutathione S-conjugates, thereby contributing to the control of cellular redox status.
• The protein localizes to the cytoplasm, consistent with its function in intracellular peptide turnover.
• Post-translational modifications identified include acetylation and phosphorylation; an alternative initiation site may generate different isoforms.
• Alternatively known as leucine aminopeptidase 3 (LAP-3) or proline aminopeptidase, reflecting its broad substrate preference.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant human LAP3 protein; the antibody may recognize both native and denatured forms. For Western blot, consider standard lysis and protein extraction methods.
• Expected bands at approximately 52–56 kDa; alternative initiation could produce multiple bands. Validate using appropriate controls.
• In immunofluorescence-paraffin (IF-P), anticipate cytoplasmic staining. Include secondary-only and isotype controls to confirm specificity.
• For ELISA applications, optimize coating antigen and antibody dilution for your specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your one-stop sourcing partner for IVD raw materials, offering LAP3 antibodies and associated reagents for studies in aminopeptidase function and glutathione metabolism. With validated antibody pairs and scalable supply, we bridge the gap from research concept to clinical application.