Anti-LAP3 (KD Validated) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P28838

Core Product Specifications and Parameters

Biological Background: LAP3 Function and Localization

• Cytosol aminopeptidase, also known as leucine aminopeptidase 3 (LAP3), leucyl aminopeptidase, proline aminopeptidase, and peptidase S (UniProt: P28838).
• Catalyzes the removal of unsubstituted N-terminal hydrophobic amino acids from various peptides, functioning as a broad-specificity aminopeptidase.
• Requires Zn²⁺ ions for catalytic activity; association with Mn²⁺ shifts substrate preference toward Cys-Gly bond hydrolysis in glutathione S-conjugates.
• Participates in glutathione metabolism and the degradation of glutathione S-conjugates, thereby contributing to the regulation of cellular redox homeostasis.
• Localized predominantly in the cytoplasm.
• Post‑translational modifications include acetylation and phosphorylation; an alternative initiation isoform exists.
• Molecular weight is approximately 56 kDa (56166 Da).

Experimental Guidance and Technical Tips

• The immunogen (aa 21–180) targets a conserved N‑terminal region; this antibody is suitable for detecting LAP3 in human, mouse, and rat samples.
• For Western blotting, optimize antibody dilution and sample loading; a band near 56 kDa is expected.
• In immunohistochemistry (IHC‑P), antigen retrieval may be required; validate staining in known positive tissues.
• As a knockdown‑validated antibody, it is appropriate for verifying LAP3 depletion in siRNA/shRNA experiments.
• For ELISA, determine optimal coating and detection antibody conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one‑stop sourcing platform for IVD raw materials. Our portfolio includes this high‑quality anti‑LAP3 rabbit monoclonal antibody, ideal for redox biology and glutathione metabolism research. We supply validated antibody pairs, recombinant proteins, bulk ancillary reagents, and tailored sourcing solutions to accelerate your assay development from concept to clinic.