Anti-LAP2 Rabbit Polyclonal Antibody for WB, ELISA - P42166/P42167

Core Product Specifications and Parameters

Biological Background: LAP2 Function and Localization

• Lamina-associated polypeptide 2 (LAP2), also known as thymopoietin (TMPO), is an inner nuclear membrane protein that exists in multiple isoforms (alpha, beta, gamma) generated by alternative splicing. The alpha isoform (this product's target) is characterized by a large N-terminal nucleoplasmic domain.
• Core function: LAP2 is involved in the structural organization of the nucleus and post-mitotic nuclear reassembly. It interacts with lamin A/C (LMNA) to anchor retinoblastoma protein (RB1) at the nuclear periphery.
• T-cell biology: The thymopoietin peptides (TP and TP5) derived from LAP2 play a role in T-cell development and function. Notably, TP5 (a pentapeptide) is an immunomodulatory molecule with therapeutic potential.
• Subcellular localization: LAP2 localizes to the nucleus and associates with chromosomes during mitosis, consistent with its role in nuclear envelope dynamics.
• Tissue distribution: Widely expressed across tissues, with highest levels in adult thymus and fetal liver, aligning with its roles in immune cell development and nuclear organization.
• Post-translational modifications: LAP2 undergoes acetylation, methylation, and phosphorylation, suggesting regulation by multiple signaling pathways.
• Additional annotations: The protein features a coiled-coil domain, DNA-binding capability, and is classified under reference proteome; mutations are associated with cardiomyopathy (CMD1T).

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-168) common to multiple LAP2 isoforms; expect detection of several bands on Western blot corresponding to different isoforms (e.g., ~38 kDa, 50 kDa, 75 kDa). Validate in the relevant cell or tissue lysate.
• For Western blot, consider using positive control samples from thymus or liver tissues where LAP2 expression is high.
• Cross-reactivity with mouse and rat has been confirmed; it is advisable to perform species-specific sequence alignment to assess epitope conservation for other species.
• When using this antibody in ELISA, determine optimal working concentrations through checkerboard titration; pairing with other detection reagents may be explored.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD development by providing one-stop access to premium raw materials. For nuclear envelope and immunology research, we offer this anti-LAP2 polyclonal antibody alongside a full portfolio of matched pairs, recombinant proteins, and ancillary reagents. Our sourcing network can also fulfill custom requests for rare targets, supporting your projects from concept to clinic.