Anti-LAMP2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P13473

Core Product Specifications and Parameters

Biological Background: LAMP2 Function and Localization

• LAMP2 (Lysosome-associated membrane glycoprotein 2), also known as CD107b, LAMPB, or LGP-96, is encoded by the LAMP2 gene and exists as three alternatively spliced isoforms: LAMP-2A, LAMP-2B, and LAMP-2C.
• Essential for lysosome biogenesis and maintenance of lysosomal pH by directly inhibiting the proton channel TMEM175, thereby facilitating lysosomal acidification for optimal hydrolase activity (PubMed:37390818).
  Related references: PMID:37390818
• Plays a critical role in chaperone-mediated autophagy (CMA) by binding substrate proteins (e.g., GAPDH, GPX4, NLRP3, MLLT11) recognized by HSPA8/HSC70 and targeting them for lysosomal degradation, a process important for protein quality control and stress responses (PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:36586411).
  Related references: PMID:11082038 PMID:18644871 PMID:24880125 PMID:36586411
• Required for the fusion of autophagosomes with lysosomes during macroautophagy, as cells lacking LAMP2 fail to accumulate STX17 on autophagosomes, preventing fusion and degradation of autophagic contents (PubMed:27628032).
  Related references: PMID:27628032
• Facilitates efficient MHC class II-mediated presentation of exogenous antigens by ensuring proper lysosomal degradation, although it is not required for endogenous antigen presentation (PubMed:15894275, PubMed:20518820).
  Related references: PMID:15894275 PMID:20518820
• Localizes to the membranes of lysosomes, late endosomes, autophagosomes, and the cell surface, consistent with its trafficking through the endocytic and autophagic pathways.
• Isoforms show tissue-specific expression: LAMP-2A is high in placenta, lung, and liver; LAMP-2B is broadly expressed; LAMP-2C is detected in intestine, heart, brain, and skeletal muscle (PubMed:26856698, PubMed:7488019).
  Related references: PMID:26856698 PMID:7488019
• Heavily glycosylated with disulfide bonds; classified as a glycoprotein and transmembrane protein. Associated with Danon disease (glycogen storage disease due to LAMP2 deficiency).

Experimental Guidance and Technical Tips

• The immunogen corresponds to the luminal domain of human LAMP2 (aa 29-375), which is highly glycosylated. Consider this when interpreting molecular weight shifts in western blot; the observed band may appear larger than the predicted 45 kDa due to extensive glycosylation.
• For western blot (WB), validate the antibody in your specific sample matrix; use reducing conditions and load appropriate controls.
• For immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval) may be required to unmask LAMP2 epitopes, especially in formalin-fixed paraffin-embedded tissues.
• In immunofluorescence (IF/ICC), LAMP2 staining should show punctate lysosomal pattern; co-staining with lysosomal markers (e.g., LAMP1) is recommended for confirmation.
• This polyclonal antibody may exhibit batch-to-batch variation; include positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement experience for IVD raw materials, providing validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents for diagnostic and research applications. This anti-LAMP2 antibody supports investigations into lysosomal biology, autophagy, and antigen presentation pathways. For bulk orders, custom formulations, or rare target sourcing, our team ensures reliable supply and technical support to accelerate your project from concept to clinic.