Anti-LAMP1/CD107a Monoclonal Antibody for Flow Cytometry - P11279

Core Product Specifications and Parameters

Biological Background: LAMP1 Function and Localization

• LAMP1 (CD107a) is a heavily glycosylated lysosomal membrane protein with a signal peptide and disulfide bonds, existing in alternatively spliced isoforms.
• Functions as a critical regulator of lysosome biogenesis, autophagy, and cholesterol homeostasis by modulating lysosomal pH through direct inhibition of the proton channel TMEM175 (PubMed:37390818). Related references: PMID:37390818
• In NK cells, LAMP1 is essential for cytotoxicity: it facilitates cytotoxic granule movement and perforin trafficking to lytic granules, and protects NK cells from degranulation-associated self-damage (PubMed:2022921, PubMed:23632890, PubMed:23847195). Related references: PMID:2022921, PMID:23632890, PMID:23847195
• Presents carbohydrate ligands to selectins, potentially mediating cell adhesion (PubMed:7685349). Related references: PMID:7685349
• Acts as a cellular receptor for Lassa virus glycoprotein, promoting viral entry and fusion in less acidic endosomes (PubMed:24970085, PubMed:25972533, PubMed:27605678, PubMed:29295909). Related references: PMID:24970085, PMID:25972533, PMID:27605678, PMID:29295909
• Supports FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed viral protein.
• Subcellular localization includes lysosome membrane, late endosome membrane, cell membrane, and cytolytic granule membrane.

Experimental Guidance and Technical Tips

• This antibody is intended for intracellular flow cytometry; for optimal detection of LAMP1 in lysosomal compartments, use fixation and permeabilization buffers suitable for intracellular antigens.
• Since LAMP1 can translocate to the cell surface during degranulation (e.g., in activated NK cells), surface staining may be observed; validate in your specific experimental system.
• If employing other applications such as western blot or immunofluorescence, preliminary testing is strongly advised, as the antibody has not been validated for those uses.
• Consider using isotype controls and titrate the antibody concentration for best signal-to-noise ratio in flow cytometry.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw material sourcing, offering a comprehensive portfolio of validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents. With this LAMP1/CD107a antibody, researchers studying lysosomal biology, autophagy, or NK cell-mediated cytotoxicity can source high-quality detection reagents alongside complementary products such as isotype controls and detection kits. Our team supports rare-target raw-material procurement and custom development to accelerate your diagnostic or research workflows from concept to clinic.