Anti-LAMP1 Polyclonal Antibody (KD Validated) for WB, IF/ICC, IHC-P, ELISA - P11279

Core Product Specifications and Parameters

Biological Background: LAMP1 Function and Localization

• LAMP1 (Lysosome-associated membrane glycoprotein 1), also known as CD107a or LGP120, is a major protein component of the lysosomal membrane, heavily glycosylated with N- and O-linked glycans that form the protective glycocalyx lining the lysosomal lumen.
• It regulates lysosomal pH by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification necessary for optimal hydrolase activity.
  Related references: PMID:37390818
• LAMP1 is involved in autophagy and cholesterol homeostasis, playing a critical role in maintaining cellular catabolic balance.
• In NK cells, LAMP1 facilitates the movement of cytotoxic granules to the cell surface, mediates perforin trafficking to lytic granules, and protects NK cells from degranulation-associated damage by their own cytotoxic contents.
  Related references: PMID:2022921, PMID:23632890, PMID:23847195
• Upon cell activation, LAMP1 can be expressed at the cell surface where it presents carbohydrate ligands to selectins, contributing to cell adhesion.
  Related references: PMID:7685349
• LAMP1 acts as a receptor for Lassa virus glycoprotein and promotes viral fusion with host membranes in less acidic endosomes, highlighting its role in microbial infection.
  Related references: PMID:24970085, PMID:25972533, PMID:27605678, PMID:29295909
• Subcellularly, LAMP1 is primarily found on lysosomal membranes, but also localizes to late endosomes, endosomes, the plasma membrane, and cytolytic granule membranes in immune cells.
• Post-translational modifications include extensive N- and O-linked glycosylation and disulfide bond formation, which are essential for its structural integrity and function.

Experimental Guidance and Technical Tips

• The immunogen for this antibody corresponds to a peptide region (aa 150-250) of human LAMP1. For Western blot, expect a band around 45 kDa, although highly variable glycosylation may cause the protein to migrate as a diffuse band at higher apparent molecular weights. Validate with appropriate positive controls.
• In immunofluorescence (IF/ICC), LAMP1 typically exhibits punctate perinuclear staining consistent with lysosomal localization. Use permeabilization and co-staining with organelle markers to confirm specificity.
• For immunohistochemistry (IHC-P), antigen retrieval and antibody titration are recommended to optimize signal-to-noise, particularly in tissues with high endogenous peroxidase activity.
• Cross-reactivity is confirmed for human, mouse, and rat, but performance in other species should be verified experimentally.
• This polyclonal antibody is suitable for ELISA; consider pairing with a monoclonal antibody for sandwich ELISA development if quantitative detection is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with streamlined access to premium IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti-LAMP1 polyclonal antibody supports research in cell biology, immuno-oncology, and infectious disease areas. For development programs requiring optimized monoclonal or polyclonal antibodies, rare-target sourcing, or scalable reagent supply, CamelBio provides tailored one-stop procurement solutions to accelerate your journey from concept to clinic.