Anti-LAMP1 Polyclonal Antibody for WB and ELISA - P11279

Core Product Specifications and Parameters

Biological Background: LAMP1 Function and Localization

• LAMP1 (Lysosome-associated membrane glycoprotein 1) is a 45 kDa type I transmembrane glycoprotein and a member of the LAMP family, characterized by a heavily glycosylated luminal domain.
• It is essential for lysosome biogenesis and autophagy, and directly regulates lysosomal lumen pH by inhibiting the TMEM175 proton channel (PubMed:37390818). Related references: PMID:37390818
• LAMP1 participates in NK-cell-mediated cytotoxicity by facilitating cytotoxic granule movement and perforin trafficking to the lytic granule (PubMed:2022921, PubMed:23632890), and protects NK cells from self-inflicted damage during degranulation (PubMed:23847195). Related references: PMID:2022921 | PMID:23632890 | PMID:23847195
• The protein presents carbohydrate ligands to selectins, contributing to cell adhesion and migration (PubMed:7685349). Related references: PMID:7685349
• During Lassa virus infection, LAMP1 acts as a host receptor for the viral glycoprotein and promotes membrane fusion in less acidic endosomes (PubMed:24970085, PubMed:25972533, PubMed:27605678, PubMed:29295909). Related references: PMID:24970085 | PMID:25972533 | PMID:27605678 | PMID:29295909
• LAMP1 also assists the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both the protease and the unprocessed viral protein.
• Subcellularly, LAMP1 is predominantly found in lysosomal and late endosomal membranes, but can also localize to the cell surface and cytolytic granule membranes upon activation.
• The protein undergoes alternative splicing and contains multiple N-glycosylation sites and disulfide bonds essential for its stability and function.

Experimental Guidance and Technical Tips

• For Western blot, consider that LAMP1 is heavily glycosylated, which may cause it to migrate at a higher apparent molecular weight (~90–120 kDa) than the predicted 45 kDa. Use appropriate protein ladders and validate band specificity with control samples.
• Optimize blocking and wash conditions to reduce potential background, as polyclonal antibodies may exhibit higher non-specific binding.
• In ELISA, validate the antibody's binding specificity and optimal dilution using recombinant LAMP1 protein or cell lysates from human samples. Pair with a suitable detection system.
• This antibody is validated for human reactivity; cross-reactivity with other species is not expected based on provided data. Confirm suitability for other species if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material provider, supplying a wide range of validated antibodies, antigens, and ancillary reagents to support diagnostic and research workflows. This anti-LAMP1 polyclonal antibody is part of our portfolio targeting lysosomal and autophagy-related proteins, offering researchers a reliable tool for studying cellular degradation pathways and viral entry mechanisms. We assist clients in sourcing rare targets and provide technical expertise to accelerate assay and kit development.