Anti-Laminin Rabbit Polyclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - Q9Y6N6 / P25391 / P24043 / P07942 / P55268 / P11047

Core Product Specifications and Parameters

Biological Background: Laminin subunit gamma-3 Function and Localization

• Laminin subunit gamma-3 (LAMC3) is a component of laminins 12, 14, and 15, which are heterotrimeric basement membrane glycoproteins.
• Laminins mediate cell attachment, migration, and organization into tissues during embryonic development by binding to cellular receptors such as integrins and interacting with other extracellular matrix components.
• LAMC3 is widely expressed in tissues including skin, heart, lung, and the reproductive tracts.
• Subcellularly, laminins are secreted and found in the extracellular matrix, specifically within the basement membrane.
• Structurally, LAMC3 contains laminin EGF-like domains, a coiled-coil region, and multiple disulfide bonds, characteristic of the laminin family.
• Genetic variants in LAMC3 are associated with disease, and the protein is annotated as a disease variant gene (UniProt keyword).
• Post-translational modifications include glycosylation, and the protein is classified as a glycoprotein.

Experimental Guidance and Technical Tips

• The antibody is raised against recombinant human Laminin protein; consider that it may recognize multiple laminin chains due to potential shared epitopes among laminin family members. Validate specificity for your target subunit in relevant models.
• Optimize antibody dilution for each application; recommended starting dilutions for WB, IF, IHC, and ELISA should be determined empirically using known positive and negative controls.
• For immunostaining, include appropriate permeabilization steps for intracellular epitopes and confirm localization against known basement membrane markers.
• Cross-reactivity with mouse and rat laminin is expected, but confirm in species-specific tissue samples if used for those species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibodies like this anti-Laminin polyclonal antibody, to support your extracellular matrix and cell adhesion research. As your one-stop sourcing partner, we provide monoclonal and polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. Whether you need scale-up supply or technical consultation, our team bridges your project from concept to clinic.