Anti-Laminin beta 1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P07942

Core Product Specifications and Parameters

Biological Background: Laminin Subunit Beta-1 Function and Localization

• Laminin subunit beta-1, also known as Laminin B1 chain, is a component of several laminin isoforms including laminin-1 (laminin-111), laminin-10, laminin-12, laminin-2, laminin-6, and laminin-8.
• Mediates cell attachment, migration, and organization during embryonic development by binding to high affinity receptors and interacting with other extracellular matrix components.
• Involved in the organization of the laminar architecture of the cerebral cortex, likely by maintaining the integrity of the basement membrane/glia limitans that anchors radial glial cell endfeet and serves as a barrier to migrating neurons (PubMed:23472759). Related references: PMID:23472759
• Required for the integrity of the basement membrane/glia limitans, which is critical for radial glial cell function in cerebral cortical development (By similarity).
• As part of laminin-1, it stimulates agrin-induced receptor clustering through a MuSK-independent pathway (By similarity).
• Localized to the extracellular matrix, basement membrane, and is secreted.
• Keywords: Basement membrane, Cell adhesion, Extracellular matrix, Glycoprotein, Phosphoprotein, Repeat.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a sequence within amino acids 1687-1786 of human Laminin beta 1. Consider sequence alignment to ensure specificity across intended species.
• For Western blotting, a band around 198 kDa is expected. Validate in cell or tissue lysates expressing Laminin beta 1.
• For immunocytochemistry/immunofluorescence, optimize fixation and permeabilization protocols to detect extracellular matrix-associated laminin.
• Cross-reactivity is reported for human, mouse, and rat; validate the antibody in the relevant species and tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with integrated access to validated IVD raw materials. This anti-Laminin beta 1 antibody, along with complementary reagents such as validated antibody pairs and ancillary extracellular matrix proteins, supports assay development for basement membrane and cell adhesion studies. For rare target sourcing or custom monoclonal antibody development, contact CamelBio to bridge your supply needs.