Anti-Lamin B2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q03252

Core Product Specifications and Parameters

Biological Background: Lamin B2 Function and Localization

• Lamin B2 is an intermediate filament protein that assembles into a filamentous meshwork, constituting the major component of the nuclear lamina on the nucleoplasmic side of the inner nuclear membrane. Related references: PMID:33033404
• It provides structural integrity to the nuclear envelope by bridging it with chromatin, and plays critical roles in nuclear assembly, chromatin organization, and telomere dynamics. Related references: PMID:33033404
• The integrity of the nuclear lamina is tightly regulated during the cell cycle, disassembling during prophase and reassembling during telophase to facilitate proper nuclear function. Related references: PMID:33033404
• Lamin B2 localizes specifically to the nuclear lamina.
• Post-translational modifications such as acetylation, phosphorylation, prenylation, and methylation are associated with Lamin B2, regulating its function and dynamics.
• Genetic variants in LMNB2 are linked to diseases including primary microcephaly and neurological disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 341–620) of human Lamin B2. Users performing sequence alignment may check conservation across species to inform cross-reactivity expectations.
• In Western blotting, a band near 70 kDa is consistent with the calculated molecular weight. Nuclear-enriched lysates may improve detection.
• For immunohistochemistry and immunofluorescence, optimized fixation and antigen retrieval protocols should be validated. A nuclear lamina staining pattern is anticipated.
• Reported cross-reactivity includes human, mouse, and rat samples; however, verification in the specific experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to IVD raw materials, supporting nuclear lamina and cell cycle research with products such as this anti-Lamin B2 antibody. Our supply capabilities include validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets—bridging your projects from concept to clinic with reliable raw-material solutions.