Anti-Lamin B1 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA, ChIP - P20700

Core Product Specifications and Parameters

Biological Background: Lamin B1 Function and Localization

• Lamin B1, encoded by the LMNB1 gene (aliases: LMN, ADLD, LMN2, LMNB, MCPH26), is a type V intermediate filament protein that constitutes a major component of the nuclear lamina.
• It assembles into a filamentous meshwork that provides structural support to the nuclear envelope and bridges the nuclear envelope and chromatin, playing a critical role in nuclear assembly, chromatin organization, and telomere dynamics.
  Related references: PMID:28716252 PMID:32910914
• The integrity of the nuclear lamina is tightly regulated during the cell cycle; Lamin B1 is involved in the disassembly of the nuclear envelope in prophase and its reformation in telophase.
  Related references: PMID:28716252 PMID:32910914
• Lamin B1 is localized specifically to the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane.
• It undergoes various post-translational modifications, including phosphorylation, acetylation, prenylation, methylation, and SUMOylation, which regulate its assembly, dynamics, and interactions.
• In sperm development, Lamin B1 is a component of the LMNB1/SUN5/SEPT12 bridge that anchors the sperm proximal centriole to the nucleus, essential for sperm head-tail coupling.
  Related references: PMID:38870534
• Mutations in LMNB1 are linked to autosomal dominant leukodystrophy (ADLD) and primary microcephaly (MCPH26), underscoring its importance in neurological development.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 397-586 at the C‑terminus of human Lamin B1; this region may recognize full‑length Lamin B1 and potentially certain splice variants. Validate specificity in your sample system.
• For Western blotting, a predominant band around 66 kDa is expected. Use appropriate loading controls and consider testing multiple tissue or cell lysates where Lamin B1 is known to be expressed.
• For immunohistochemistry and immunofluorescence, optimize permeabilization and antigen retrieval conditions, as the nuclear lamina may require specific fixation protocols.
• In chromatin immunoprecipitation (ChIP) assays, confirm enrichment with positive and negative control genomic loci associated with the nuclear lamina.
• For immunoprecipitation, include pre‑immune serum or isotype control and verify capture by Western blot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one‑stop partner for IVD raw‑material sourcing, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material procurement. This Lamin B1 polyclonal antibody supports research into nuclear architecture, chromatin organization, and cell cycle dynamics. Our integrated supply chain and technical support help move your diagnostic development from concept to clinic smoothly.