Anti-Lamin A/C Polyclonal Antibody for WB, IF/ICC, IHC-P, ChIP, ELISA - P02545

Core Product Specifications and Parameters

Biological Background: Lamin A/C Function and Localization

• Lamins are intermediate filament proteins that assemble into the nuclear lamina, providing structural support and regulating nuclear assembly, chromatin organization, and telomere dynamics.
  Related references: PMID:10080180, PMID:10580070, PMID:10587585
• Lamin A and C regulate matrix stiffness and confer nuclear mechanical properties, essential for tissue mechanics.
  Related references: PMID:23990565, PMID:25127216
• The cell cycle strictly controls lamina integrity via phosphorylation-dependent disassembly during prophase and reassembly in telophase.
  Related references: PMID:2188730, PMID:2344612
• In DNA repair, lamin A/C is recruited by XRCC4 and IFFO1 to double-strand breaks to immobilize broken DNA ends and prevent chromosome translocation.
  Related references: PMID:31548606
• Lamin A/C is essential for skeletal muscle, cardiac, and peripheral nervous system development, and for muscle satellite cell proliferation.
  Related references: PMID:10080180, PMID:10814726, PMID:11799477, PMID:10580070, PMID:12927431, PMID:18611980
• It is also required for osteoblastogenesis, bone formation, and prevents fat infiltration in muscle and bone marrow.
  Related references: PMID:12075506, PMID:15317753, PMID:18611980, PMID:10587585
• In aging and vascular disease, prelamin-A accumulates in vascular smooth muscle cells, inducing senescence via mitotic disruption and DNA damage.
  Related references: PMID:20458013
• Lamin A/C predominantly localizes to the nuclear lamina, but also to the nucleoplasm, nuclear matrix, and nuclear speckles.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 400–500 of human lamin A/C) lies within the tail domain conserved between lamin A and lamin C; the antibody is expected to recognize both isoforms.
• For Western blotting, run reduced samples and expect a major band around 74 kDa; lamin C may appear slightly lower.
• In immunofluorescence or immunohistochemistry, anticipate nuclear envelope and nucleoplasmic staining patterns; include isotype or peptide competition controls.
• For ChIP, optimize crosslinking and shearing conditions, as lamin A/C associates with large chromatin domains.
• For ELISA, consider using recombinant lamin A/C protein as a standard and verify cross-reactivity with your species of interest.
• Lot-to-lot consistency should be verified for critical quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and technical support, streamlining your development from concept to clinic. For nuclear envelope and lamin research, CamelBio can supply validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents, as well as assist in sourcing rare or custom targets. Partner with CamelBio to accelerate your diagnostic assay development with reliable, ready-to-use raw materials.