Anti-Lamin A/C Polyclonal Antibody for WB, IF/ICC, IHC-P, ChIP, ELISA - P02545

Core Product Specifications and Parameters

Biological Background: Lamin A/C Function and Localization

• Lamin A and C (splice isoforms of prelamin-A/C) are intermediate filament proteins that form the nuclear lamina meshwork, providing structural support to the nuclear envelope and linking chromatin to the nuclear periphery (PubMed:10080180, PubMed:10580070, PubMed:10587585). They also regulate matrix stiffness and nuclear mechanical properties (PubMed:23990565, PubMed:25127216). Related references: PMID:10080180, PMID:10580070, PMID:10587585, PMID:23990565, PMID:25127216
• The nuclear lamina undergoes cyclic disassembly and reassembly during mitosis, regulated by phosphorylation, ensuring proper nuclear division (PubMed:2188730, PubMed:2344612). Related references: PMID:2188730, PMID:2344612
• Lamin A/C participates in DNA repair by immobilizing broken DNA ends via recruitment by XRCC4 and IFFO1, preventing chromosome translocations (PubMed:31548606). Related references: PMID:31548606
• Essential for normal development, lamins are required for peripheral nervous system and skeletal muscle differentiation, muscle satellite cell proliferation, osteoblastogenesis, and bone formation (PubMed:10080180, PubMed:10814726, PubMed:11799477, PubMed:12075506, PubMed:15317753, PubMed:18611980). Related references: PMID:10080180, PMID:10814726, PMID:11799477, PMID:12075506, PMID:15317753, PMID:18611980
• In cardiac muscle, lamin A/C is critical for homeostasis; mutations cause dilated cardiomyopathy and other cardiopathies (PubMed:10580070, PubMed:12927431, PubMed:18611980). Related references: PMID:10580070, PMID:12927431, PMID:18611980
• Accumulation of prelamin-A/C in vascular smooth muscle cells accelerates senescence by inducing mitotic failure and DNA damage, contributing to vascular aging (PubMed:20458013). Related references: PMID:20458013
• Subcellularly, lamin A/C localizes predominantly to the nuclear lamina, but is also found in the nucleoplasm, nuclear matrix, and nuclear speckles, reflecting its diverse nuclear functions.
• Post-translational modifications (acetylation, phosphorylation, methylation, prenylation, SUMOylation) and tissue-specific regulation (e.g., age-related upregulation in arterial smooth muscle) control lamina dynamics and function.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 403-572, a conserved region of human Lamin A/C, suggesting potential cross-reactivity with mouse and rat orthologs. Therefore, this antibody may be suitable for detecting Lamin A/C in these species; however, users should validate performance in their specific experimental systems.
• Based on the listed applications, this polyclonal antibody can be tested in Western blot, immunofluorescence/immunocytochemistry, immunohistochemistry-paraffin, chromatin immunoprecipitation, and ELISA. Optimal working dilutions and conditions should be determined empirically.
• For ChIP assays, consider the reported involvement of Lamin A/C in chromatin organization and DNA repair; proper fixation and shearing protocols are critical.
• The predicted molecular weight is 74 kDa, but both lamin A and C may appear as distinct bands or a doublet in WB, as they are alternatively spliced isoforms with slight size differences.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD developers and researchers, offering one-stop access to validated raw materials, technical services, and consulting from concept to commercialization. This anti-Lamin A/C polyclonal antibody exemplifies our commitment to providing high-quality reagents for nuclear lamina biology and laminopathy research. Our supply portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target procurement to meet your diagnostic manufacturing needs.