Anti-Lamin A/C Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P02545

Core Product Specifications and Parameters

Biological Background: Lamin A/C Function and Localization

• Lamin A and C are major components of the nuclear lamina, a filamentous meshwork underlying the inner nuclear membrane, and are encoded by the LMNA gene through alternative splicing.
• They provide structural support for the nuclear envelope, bridge chromatin and the nuclear envelope, and regulate nuclear assembly, chromatin organization, and telomere dynamics. Related references: PMID:10080180, PMID:10580070, PMID:10587585
• Lamins play a role in DNA repair: Lamin A/C is recruited to DNA double-strand breaks by XRCC4 and IFFO1, immobilizing broken DNA ends to prevent chromosome translocations. Related references: PMID:31548606
• Lamin A/C regulates matrix stiffness by conferring nuclear mechanical properties, influencing cell migration and differentiation. Related references: PMID:23990565, PMID:25127216
• Subcellular localization includes nucleus lamina, nuclear envelope, nucleoplasm, nuclear matrix, and nuclear speckles.
• Post-translational modifications such as prenylation, methylation, phosphorylation, acetylation, and isopeptide bond formation regulate lamin assembly and function.
• Tissue-specifically, prelamin-A/C accumulates in aged vascular smooth muscle cells and atherosclerotic lesions, contributing to cellular senescence. Related references: PMID:20458013
• Mutations in LMNA cause laminopathies, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy, lipodystrophy, and progeria syndromes.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 403–572, corresponding to the C-terminal tail domain common to lamin A and C, which is less conserved among intermediate filament proteins, suggesting high specificity.
• For Western blot, the predicted molecular weight is ~74 kDa for lamin A and ~65 kDa for lamin C; both bands may be detected. Validate using nuclear extracts from human, mouse, or rat cell lines.
• For IHC-P and IF/ICC, nuclear envelope and nucleoplasmic staining is expected. Optimization of antigen retrieval and permeabilization may be required for formalin-fixed tissues.
• For IP and ELISA, the antibody may be used to enrich lamin A/C complexes; however, blocking and wash conditions should be empirically determined.
• Given the KO validation, this antibody is suitable for confirming lamin A/C depletion and studying loss-of-function phenotypes in nuclear organization and DNA repair assays.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide a seamless supply chain for high-quality IVD raw materials, including validated monoclonal antibodies like this Lamin A/C antibody. Whether you need ready-to-use antibody pairs, bulk reagent lots, or customized sourcing for nuclear lamina and laminopathy research, our platform connects you with reliable solutions to accelerate your diagnostic development.