Anti-LAMC2 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA - Q13753

Core Product Specifications and Parameters

Biological Background: Laminin Subunit Gamma-2 Function and Localization

• Laminin subunit gamma-2 is a component of laminin-5 (laminin 332), a basement membrane protein that mediates cell attachment, migration, and tissue organization during embryonic development.
• It is known under multiple aliases, including cell-scattering factor (CSF) 140 kDa subunit, epiligrin subunit gamma, kalinin subunit gamma, nicein subunit gamma, and ladsin 140 kDa subunit.
• The encoded protein, ladsin, exerts cell-scattering activity toward a wide variety of cells, including epithelial, endothelial, and fibroblastic cells, indicating a role in cell motility.
• The protein is secreted and localized to the extracellular matrix, specifically within the basement membrane.
• Alternative splicing produces isoforms; the large variant is expressed only in specific epithelial cells of embryonic and neonatal tissues, while the small variant is found in cerebral cortex, lung, and distal tubules of kidney in 17-week embryos.
• It contains laminin EGF-like domains, coiled-coil regions, and disulfide bonds, and is a glycoprotein with heparin-binding capability.
• Mutations in LAMC2 are associated with junctional epidermolysis bullosa, highlighting its critical function in epithelial adhesion and skin integrity.

Experimental Guidance and Technical Tips

• The immunogen targets amino acids 746-860 near the C-terminus of human LAMC2, which may help detect full-length protein and certain isoforms; cross-reactivity with mouse and rat has been observed, but sequence alignment is recommended for other species.
• In Western blot, the predicted molecular weight is approximately 131 kDa; note that glycosylation or proteolytic processing can yield additional bands, so validate with appropriate controls.
• For immunofluorescence applications (IF/ICC, IF-P), optimize permeabilization and fixation conditions, and include positive and negative control samples to confirm specific staining.
• When used in ELISA, consider pairing with a suitable capture or detection antibody; optimal working concentrations must be empirically determined for each assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. For LAMC2, we offer comprehensive supply solutions to support basement membrane and cell adhesion research, facilitating your development of robust diagnostic assays.