Anti-LAMC2 Monoclonal Antibody for WB, IF/ICC, ELISA - Q13753

Core Product Specifications and Parameters

Biological Background: LAMC2 Function and Localization

• LAMC2 encodes the gamma-2 subunit of laminin-5 (also known as kalinin, epiligrin, nicein, or ladsin), a key component of the basement membrane extracellular matrix.
• The protein mediates cell attachment, migration, and tissue organization during embryonic development by interacting with other extracellular matrix components and cell surface receptors.
• Ladsin, a proteolytic fragment of laminin gamma-2, exerts potent cell-scattering activity toward epithelial, endothelial, and fibroblastic cells.
• LAMC2 is secreted and deposited in the extracellular matrix, specifically localized to the basement membrane zone where epithelial tissues contact connective stroma.
• Tissue expression is tightly regulated; the large splice variant appears primarily in embryonic and neonatal epithelial cells, while the small variant is detected in fetal cerebral cortex, lung, and kidney distal tubules.
• Structural features include laminin EGF-like repeats, a coiled-coil domain, multiple disulfide bonds, and glycation sites, which contribute to its adhesive and signaling properties.
• Mutations in LAMC2 are associated with junctional epidermolysis bullosa, a group of inherited skin fragility disorders.
• Key attributes include alternative splicing, heparin-binding, proteoglycan-like glycosylation, and a molecular weight of approximately 131 kDa (predicted 130,976 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 746–860, a region within the C-terminal portion of the full-length protein. Consider that the smaller splice variants may lack this epitope; validate target detection in your sample type.
• For Western blot, a starting dilution of 1:1000–1:2000 is typical for monoclonal antibodies; adjust based on signal. Bands at ~100 kDa, ~131 kDa, and ~140 kDa may appear due to alternative splicing and proteolytic processing.
• In IF/ICC, permeabilize cells if intracellular staining is needed, and include a positive control known to express LAMC2, such as keratinocytes or basement membrane-containing tissues.
• For ELISA, this antibody can be used as a capture or detection reagent; perform checkerboard titration to determine optimal working concentrations for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a broad portfolio of IVD raw materials, including validated antibody pairs, recombinant monoclonal antibodies, and ancillary reagents. For studies focused on cell adhesion, extracellular matrix biology, or basement membrane disorders, this anti-LAMC2 monoclonal antibody supports reliable detection. Our platform simplifies the procurement of bulk orders, customized formulations, and rare-target raw materials, helping you accelerate your diagnostic development pipeline.