Anti-LAMC1 Rabbit Polyclonal Antibody for WB, IHC-P, and ELISA - P11047

Core Product Specifications and Parameters

Biological Background: LAMC1 Function and Localization

• LAMC1 encodes laminin subunit gamma-1, a major component of laminin-1 (laminin-111), which also includes alpha-1 and beta-1 chains.
• As part of laminin-1, it mediates cell attachment, migration, and organization into tissues during embryonic development by interacting with other extracellular matrix components.
• It is involved in agrin-induced acetylcholine receptor clustering via a MuSK-independent pathway, important for neuromuscular junction formation.
• LAMC1 is a member of the laminin family, a group of heterotrimeric glycoproteins found in basement membranes.
• Subcellularly, laminin gamma-1 is secreted and localized to the extracellular matrix and basement membrane.
• The protein contains laminin EGF-like domains, coiled-coil regions, disulfide bonds, and is a phosphoprotein and glycoprotein.
• It is expressed in the basement membranes of various tissues, serving as a major structural component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1030-1275 near the C-terminus; it may be suitable for detecting full-length laminin gamma-1 in denaturing and native conditions.
• For Western blot, the predicted molecular weight is ~178 kDa. Consider using appropriate gel percentage and transfer conditions (e.g., extended transfer time or wet transfer) to ensure efficient blotting of high-molecular-weight proteins.
• For IHC-P, antigen retrieval may be required; validate the antibody on human, mouse, or rat tissues known to have basement membrane structures (e.g., kidney, placenta).
• In ELISA, the antibody can serve as a capture or detection reagent. Titration and pairing optimization with a matched antibody are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing high-quality antibodies like this anti-LAMC1 polyclonal antibody to support extracellular matrix and laminin-related research. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your diagnostic development from concept to clinic.