Anti-LAMC1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P11047

Core Product Specifications and Parameters

Biological Background: LAMC1 Function and Localization

• Laminin subunit gamma-1 (LAMC1), also known as laminin B2 chain or S-LAM gamma, is a component of several laminin isoforms, including laminin-1, laminin-2, and laminin-10, among others.
• Laminins are key extracellular matrix glycoproteins that mediate cell attachment, migration, and tissue organization during embryonic development by interacting with integrins and other matrix components.
• As part of laminin-1 (laminin-111), LAMC1 contributes to the stimulation of agrin-induced acetylcholine receptor clustering at the neuromuscular junction via a MuSK-independent pathway.
• LAMC1 is predominantly localized to the basement membranes of various tissues, where it forms a structural scaffold essential for tissue integrity and signaling.
• It contains typical laminin domains, including laminin EGF-like repeats, coiled-coil regions, and multiple disulfide bonds, contributing to its structural stability.
• Post-translational modifications include glycosylation and phosphorylation, which may regulate laminin function and interactions.
• LAMC1 is part of the laminin family, encoded by the LAMC1 gene (formerly known as LAMB2) in humans.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1030-1275 in the C-terminal region of human LAMC1, suggesting the antibody likely recognizes the full-length protein and possibly the gamma-1 chain in laminin complexes.
• For Western blotting, given the high molecular weight (~178 kDa), consider using low-percentage SDS-PAGE gels (e.g., 6-8%) and extended transfer times with appropriate buffer conditions.
• When performing immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval) may be necessary to expose the epitope, and optimal antibody dilution should be determined empirically.
• The antibody's cross-reactivity with mouse and rat makes it suitable for translational studies using rodent models; validate performance in your specific tissue of interest.
• For ELISA applications, ensure the recombinant protein or coated antigen presents the correct epitope region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to deliver comprehensive IVD raw material sourcing, from discovery through clinical application. This anti-LAMC1 monoclonal antibody supports investigations into basement membrane biology and laminin-related pathologies. Beyond individual antibodies, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target reagents to streamline your assay development pipeline.