Anti-Lambda Light Chain Monoclonal Antibody for WB, IHC-P, ELISA - P01701

Core Product Specifications and Parameters

Biological Background: Lambda Light Chain Function and Localization

• IGLV1-51 encodes the variable region of lambda immunoglobulin light chains, critical for antigen recognition. Related references: PMID:24600447
• Membrane-bound forms serve as B‑cell receptors initiating clonal expansion; secreted forms mediate humoral elimination of antigens. Related references: PMID:20176268, PMID:22158414
• Antigen-binding sites are formed by pairing with heavy chain variable domains; V‑(D)‑J rearrangement and somatic hypermutation drive affinity maturation. Related references: PMID:17576170, PMID:20176268
• The protein localizes to the cell membrane (as part of BCR) and is secreted as antibody component.
• Structural features include a signal peptide, intra-domain disulfide bonds, and an N‑terminal pyrrolidone carboxylic acid cyclization.
• Together with J segments, it contributes to the combinatorial diversity of the antibody repertoire.

Experimental Guidance and Technical Tips

• The immunogen covers the full variable domain (aa 1–117) of human IGLV1-51, suggesting the antibody can recognize native light chains in denaturing and native conditions.
• In Western blotting of reduced samples, anticipate a band at approximately 25 kDa for the lambda light chain; a 12 kDa band may appear if processing truncations or free variable domains are present.
• For immunohistochemistry (paraffin), antigen retrieval and antibody dilution should be optimized; known cross-reactivity with mouse and rat tissues can be useful for translational studies.
• The antibody is suitable for ELISA; consider coating with recombinant protein or antigen-specific capture setups to validate binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutions with a seamless supply chain for IVD raw materials. For immunoglobulin light chain research and assay development, we offer validated monoclonal antibodies, optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets. From concept to clinic, CamelBio streamlines your path with comprehensive technical services and reliable material supply.