Anti-LAMB3 Rabbit Polyclonal Antibody for WB, ELISA - Q13751

Core Product Specifications and Parameters

Biological Background: LAMB3 Function and Localization

• Laminin subunit beta-3 (LAMB3), also known as epiligrin subunit beta, kalinin B1 chain, or BM600-125KDA, is a large glycoprotein and a component of laminin-5 (laminin-332), a major basement membrane protein.
• LAMB3 mediates cell adhesion, migration, and tissue organization during embryonic development by binding to integrin receptors and interacting with other extracellular matrix components.
• As part of the laminin family, LAMB3 heterotrimerizes with alpha-3 and gamma-2 chains to form laminin-5, which is essential for the structural integrity of the basement membrane.
• The protein is secreted and localized to the extracellular matrix, specifically in the basement membrane zone of stratified epithelia, where it anchors epithelial cells to the underlying connective tissue.
• LAMB3 contains multiple laminin EGF-like domains and coiled-coil regions, and its structure is stabilized by disulfide bonds; the full-length protein has a molecular weight of approximately 130 kDa.
• Genetic mutations in LAMB3 are linked to junctional epidermolysis bullosa, a severe skin fragility disorder, highlighting its critical role in dermal-epidermal adhesion.
• The protein is subject to post-translational modifications including N-linked glycosylation, which is important for its folding and function.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to the C-terminal region (amino acids 762-1004) of human LAMB3; therefore, consider that the antibody may preferentially recognize this epitope in your assays.
• For Western blot (WB), LAMB3 is detected as a ~130 kDa band under reducing conditions; validate the band specificity in your sample type and include appropriate molecular weight markers.
• In ELISA applications, this antibody can be used for detecting LAMB3 in human samples, and cross-reactivity with rat LAMB3 allows for broader experimental applications; however, titration of the antibody is recommended for optimal signal.
• Due to the presence of disulfide bonds, reducing agents in sample loading buffer may be necessary for full denaturation of the protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers one-stop access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers focusing on extracellular matrix biology, cell adhesion, or skin disorders, the LAMB3 antibody is one of many high-quality tools available to accelerate your diagnostic development from concept to clinic. Let CamelBio support your project with reliable sourcing and technical consultation tailored to your needs.