Anti-LAMA4 Rabbit Polyclonal Antibody for WB, ELISA - Q16363

Core Product Specifications and Parameters

Biological Background: LAMA4 Function and Localization

• Laminin subunit alpha-4 (LAMA4) is a large extracellular glycoprotein that serves as an alpha chain component of laminin-8 (α4β1γ1), laminin-9 (α4β2γ1), and laminin-14 (α4β2γ3) isoforms.
• LAMA4 mediates cell attachment, migration, and tissue organization during embryonic development via high-affinity integrin receptor interactions within the extracellular matrix.
• Belongs to the laminin family of basement membrane proteins, characterized by EGF-like domains, coiled-coil regions, and multiple disulfide bonds contributing to structural stability.
• Localized to the basement membrane and extracellular matrix; secreted from various mesenchymal and fibroblast cell types.
• Post-translationally modified as a proteoglycan and glycoprotein, with alternative splicing generating multiple isoforms with distinct tissue distributions.
• In adult tissues, LAMA4 is strongly expressed in heart, lung, ovary, intestines, placenta, and liver, with low levels in skeletal muscle, kidney, brain, and other tissues; high fetal expression in lung and kidney.
• Genetic variants in LAMA4 are associated with cardiomyopathy (CMD1JJ), highlighting its critical role in cardiac muscle function and integrity.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight of LAMA4 is approximately 200–203 kDa; use gradient gels (e.g., 4–12%) and extended transfer times or wet transfer conditions to optimize high-molecular-weight protein detection.
• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1–100) of human LAMA4; consider using the immunizing peptide as a positive control in ELISA-based assays to confirm antibody specificity.
• As a polyclonal antibody, this product may exhibit lot-to-lot variability; preliminary titration and validation in the relevant human tissue or cell lysate are recommended for each new batch.
• This antibody has been validated for human reactivity only; cross-reactivity with other species (e.g., mouse, rat) has not been tested and should be verified experimentally if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive one-stop sourcing platform for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and specialized rare-target raw-material procurement. With this LAMA4 polyclonal antibody, we support your laminin and extracellular matrix research needs, ensuring seamless access to high-quality reagents. Our team stands ready to assist with customized sourcing and technical consulting to advance your projects from concept to clinical application.