Anti-LALBA Polyclonal Antibody for WB, IHC-P, ELISA - P00709

Core Product Specifications and Parameters

Biological Background: Alpha-lactalbumin Function and Localization

• LALBA encodes alpha-lactalbumin, also known as lactose synthase B protein and lysozyme-like protein 7 (LYZL7), a member of the glycoside hydrolase family.
• It functions as the regulatory subunit of lactose synthase, altering the substrate specificity of galactosyltransferase in the mammary gland to favor glucose, enabling lactose synthesis, the major carbohydrate in milk.
• In other tissues, galactosyltransferase transfers galactose onto N-acetylglucosamine of oligosaccharide chains in glycoproteins, without alpha-lactalbumin involvement.
• Alpha-lactalbumin is secreted and specifically expressed in the mammary gland; it is a major milk protein.
• The protein contains key structural features including three-dimensional structure, calcium and zinc binding sites, disulfide bonds, and glycosylation.
• It is classified under keywords: lactose biosynthesis, milk protein, secreted, signal, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20-142 of human LALBA, which excludes the signal peptide; this design may enhance specificity for the mature protein.
• For Western blot (WB), a band at approximately 16 kDa is expected; consider using appropriate percentage gels to resolve small proteins.
• In immunohistochemistry (IHC-P), validate antibody performance in relevant mammary gland tissues across human, mouse, and rat, as cross-reactivity is reported.
• ELISA applications may benefit from using recombinant protein as a standard; consider optimizing coating and detection conditions.
• Due to cross-reactivity with mouse and rat, this antibody can be utilized for comparative studies in rodent models of lactation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials, including validated polyclonal antibodies like this anti-LALBA antibody. For lactose biosynthesis and mammary gland biology projects, we offer one-stop sourcing of antibody pairs, bulk ancillary reagents, and customized procurement for rare target materials, streamlining your development from concept to clinic.