Anti-Ladinin-1 (LAD1) Rabbit Polyclonal Antibody for WB and ELISA - O00515

Core Product Specifications and Parameters

Biological Background: Ladinin-1 (LAD1) Function and Localization

• Ladinin-1 (LAD1), also known as Linear IgA disease antigen (LADA), is a 517 amino acid protein encoded by the LAD1 gene.
• It functions as an anchoring filament protein and is a critical component of the basement membrane zone, promoting dermal-epidermal adhesion.
• Subcellularly, LAD1 is secreted and localizes to the extracellular space, specifically within the basement membrane of the extracellular matrix.
• UniProt keywords include Basement membrane, Extracellular matrix, Methylation, and Phosphoprotein, indicating post-translational modifications that may regulate its function.
• The protein contains repeat regions characteristic of structural extracellular matrix proteins, potentially mediating interactions with other basement membrane components.

Experimental Guidance and Technical Tips

• The immunogen, spanning amino acids 40–200 of human LAD1, is likely conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blotting, consider using whole-cell lysates or tissue extracts enriched in basement membrane proteins (e.g., skin, kidney) where LAD1 is abundantly expressed.
• Validate the antibody in your specific sample system, as endogenous expression levels and potential post-translational processing may influence detection.
• When performing ELISA, pairing this polyclonal antibody with a monoclonal detection antibody can improve specificity; always include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-LAD1 antibody supports investigations into basement membrane biology, extracellular matrix disorders, and autoimmune conditions such as linear IgA disease. Our comprehensive supply capabilities include validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets to streamline your assay development.