Anti-Lactyl-Histone H4 (Lys5) Rabbit Monoclonal Antibody (P62805) for WB, IHC-P, ELISA

Core Product Specifications and Parameters

| Product Name | Lactic acid-Histone H4-K5 Rabbit mAb | | Remarks/Alias | H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; H4C16; HIST2H4; HIST2H4A; Lactic acid -Histone H4-K5 | | Species | Human | | GeneID (human) | 8359 | | GeneID | 8359 | | Immunogen | Synthetic Peptide|A synthetic lactylated peptide around K5 of human Histone H4 (NP_003539.1). | | Source | Rabbit | | Category | Acetylated Antibodies | | Application | WB, IHC-P, ELISA | | Cross Reactivity | Human, Mouse, Rat, Other (Wide Range Predicted) | | SWISS | P62805 | | Protein Weight | 11kDa | | Shipping | Ice bag |

Biological Background: Histone H4 Function and Localization

Histone H4 is a core component of the nucleosome, the fundamental repeating unit of chromatin. Nucleosomes wrap and compact DNA, restricting access to the genetic material and thereby playing a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Related references: UniProt P62805

Histone H4 is subject to a complex array of post-translational modifications (PTMs), including acetylation, methylation, phosphorylation, ubiquitination, and various lysine acylations. These modifications constitute a 'histone code' that regulates chromatin structure and gene expression. Specifically, lysine 5 (K5) can be modified by acetylation, crotonylation, butyrylation, propionylation, and lactylation. Lactylation at K5 has recently emerged as a novel epigenetic mark that links cellular metabolism to gene regulation. Related references: PMID:31645732

Subcellular localization studies indicate that Histone H4 is predominantly found in the nucleus and associated with chromosomes. Related references: UniProt P62805

Experimental Guidance and Technical Tips

• The immunogen is a synthetic lactylated peptide encompassing Lys5 of human Histone H4, designed to ensure specificity for the lactyl-lysine modification. When performing Western blot (WB), consider that endogenous lactylation levels can be low; optimal detection may require loading higher amounts of nuclear-enriched lysates or treating cells with lactate or glycolysis modulators.
• For immunohistochemistry (IHC-P), antigen retrieval and permeabilization protocols should be carefully optimized. Staining intensity may vary with metabolic activity in tissues. Use appropriate negative controls, such as peptide competition, to confirm signal specificity.
• In ELISA, this antibody can be used to detect lactylated Histone H4 peptides or proteins. As with other modification-specific antibodies, validation against non-lactylated or differently acylated peptides is recommended to verify selectivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, connecting diagnostic developers with high-quality, validated antibodies like this anti-lactyl Histone H4 (K5) rabbit monoclonal antibody. We supply optimized monoclonal and polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and rare target raw materials to support your assay development from concept to clinic. For your epigenetic and chromatin research or IVD kit needs, CamelBio ensures reliable access to critical reagents.