Anti-Lactyl-Histone H4 (K5) Rabbit Polyclonal Antibody (P62805) for WB, DB, ELISA

Core Product Specifications and Parameters

Biological Background: Histone H4 Function and Localization

Histone H4 is a core component of the nucleosome, wrapping and compacting DNA into chromatin to regulate access for transcription, DNA repair, replication, and chromosomal stability. The dynamic modulation of chromatin structure is governed by a complex set of post-translational modifications (PTMs), collectively termed the "histone code." Related references: UniProt P62805

Lactylation of histone H4 at lysine 5 (corresponding to K6 in UniProt numbering) has been identified as a novel epigenetic mark linking cellular metabolism to gene regulation. This modification was characterized in macrophages and tumor cells, where it is induced by lactate accumulation and contributes to the activation of reparative gene expression programs PMID:31645732. Additional lactylation sites have been mapped to K9, K13, K17, K32, K78, and K92, underscoring the widespread role of this acylation in chromatin signaling PMID:31645732, PMID:37720100.

Histone H4 localizes primarily to the nucleus and is integrated into chromosomes throughout the cell cycle. Acetylated H4 is enriched in the nuclei of maturing spermatids UniProt P62805.

Beyond lactylation, histone H4 undergoes extensive and combinatorial modification, including acetylation, methylation, phosphorylation, crotonylation, succinylation, and butyrylation at multiple residues, each contributing to distinct chromatin states and functional outcomes. Key modification sites and their modifying enzymes have been extensively cataloged. Related references: PMID:17967882, PMID:2474456, PMID:7664735, PMID:21925322, PMID:22389435, PMID:27105113.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic lactylated peptide encompassing lysine 5 of human histone H4. For optimal specificity, consider using a non-lactylated peptide or pre-adsorption controls in your assay.
• This antibody is reported for Western blot (WB), dot blot (DB), and ELISA. For WB, a typical band is expected at ~11 kDa. Detection of endogenous lactylated histone H4 may require enrichment or optimization of lysis conditions due to low stoichiometry.
• The predicted wide cross-reactivity (Human, Mouse, Rat, and others) suggests conserved epitope sequences across species. Nevertheless, species-specific validation is recommended.
• When using this antibody in ELISA or dot blot, confirm coating efficiency with positive control lactylated antigens and consider blocking with lactylated BSA to reduce background.
• For chromatin-related applications, histone extraction protocols (acid extraction or salt extraction) are advisable to enrich for tagged histones.
• Always validate the antibody's performance in the relevant sample system, as post-translational modifications are context-dependent and can vary with cell type and metabolic state.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw-material sourcing, offering this anti-lactyl-histone H4 (K5) rabbit polyclonal antibody to support epigenetic research and diagnostic development. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents tailored to your assay requirements. From conventional targets to rare modifications, CamelBio bridges the gap between concept and clinic with reliable supply solutions and technical support.