Anti-LACTB Rabbit Polyclonal Antibody for WB, ELISA - P83111

Core Product Specifications and Parameters

Biological Background: LACTB Function and Localization

• LACTB (serine beta-lactamase-like protein LACTB, mitochondrial) is a mitochondrial serine protease encoded by the LACTB gene; it belongs to the beta-lactamase superfamily, and alternative splicing generates isoforms with molecular weights of ~41 kDa and ~60 kDa (as indicated by the SWISS record).
• It functions as a regulator of mitochondrial lipid metabolism by reducing PISD protein levels, an enzyme that converts phosphatidylserine to phosphatidylethanolamine, thus modulating mitochondrial membrane composition. Related references: PMID:28329758
• LACTB also acts as a tumor suppressor, inhibiting the proliferation of multiple breast cancer cell lines through its effects on mitochondrial lipid metabolism. Related references: PMID:28329758
• Subcellularly, LACTB localizes to the mitochondrion, consistent with its role in organellar lipid regulation.
• It is predominantly expressed in skeletal muscle, but its presence in other tissues may relate to its tumor‑suppressive functions outside muscle.
• Post‑translational modifications include acetylation, which may influence its stability or activity; the protein also contains a transit peptide for mitochondrial import.
• Classified by UniProt keywords as a hydrolase, protease, lipid metabolism protein, and tumor suppressor, LACTB is a multifaceted enzyme with implications for both metabolic regulation and oncogenic pathways.

Experimental Guidance and Technical Tips

• For Western blot, the antibody likely detects both major isoforms (41 kDa and 60 kDa) due to the use of a recombinant immunogen spanning a central region (aa 116–255) that is present in all described transcripts; verify band patterns in the relevant species and tissue, especially skeletal muscle.
• ELISA performance may be optimized by using the same immunogen as a coating antigen; consider testing with recombinant LACTB protein or cell lysates from LACTB-expressing systems.
• As a polyclonal antibody, it may recognize multiple epitopes, which could yield robust signals but also requires careful titration and inclusion of appropriate controls.
• Cross-reactivity with mouse and rat suggests suitability for studies using rodent models; however, confirm specificity via knockdown or knockout validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For LACTB research in areas such as mitochondrial function and tumor suppression, we can source validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support your assay development and biomarker discovery. Leverage our integrated supply network for rare target raw materials tailored to your production needs.