Anti-L3MBTL3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q96JM7

Core Product Specifications and Parameters

Biological Background: L3MBTL3 Function and Localization

• L3MBTL3 (Lethal(3)malignant brain tumor-like protein 3), also known as MBT-1, is encoded by the L3MBTL3 gene (KIAA1798, MBT1) in humans.
• It functions as a negative regulator of Notch target gene expression, required for RBPJ-mediated transcriptional repression. Related references: PMID:29030483
• L3MBTL3 recruits the histone demethylase KDM1A to Notch-responsive elements, promoting KDM1A-mediated H3K4me demethylation. Related references: PMID:29030483
• It is involved in the regulation of ubiquitin-dependent degradation of methylated non-histone proteins, including SOX2, DNMT1, and E2F1, by acting as an adaptor that recruits the CRL4-DCAF5 E3 ubiquitin ligase complex to methylated target proteins. Related references: PMID:29691401 PMID:30442713
• Required for normal maturation of myeloid progenitor cells (By similarity).
• Subcellular localization is primarily nuclear.
• The protein features zinc-finger domains and is classified as a chromatin regulator; it is subject to phosphorylation and ubiquitination (isopeptide bond formation).
• Alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blot, validate the antibody in lysates from human or mouse cell lines; the predicted molecular weight is ~88 kDa.
• For immunohistochemistry (IHC-P), consider antigen retrieval and test on paraffin-embedded tissues expressing L3MBTL3.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization conditions suitable for nuclear targets.
• For ELISA, use the recombinant immunogen (amino acids 1-255) as a positive control to assess antibody reactivity.
• As a polyclonal antibody, lot-to-lot variation may occur; always include appropriate controls and validate in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio provides antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents for chromatin biology and Notch signaling research. For L3MBTL3, we supply this validated rabbit polyclonal antibody and can assist in sourcing rare-target reagents and bulk custom orders to support your diagnostic development needs.