Anti-L3MBTL2 Polyclonal Antibody for WB, ELISA - Q969R5

Core Product Specifications and Parameters

Biological Background: L3MBTL2 Function and Localization

• L3MBTL2 (Lethal(3)malignant brain tumor-like protein 2), also known as L3MBT and H-l(3)mbt-l, is encoded by the L3MBTL2 gene (GeneID: 83746) in humans.
• Putative Polycomb group (PcG) protein that maintains the transcriptionally repressive state of genes, likely through chromatin modification, contributing to heritable changes in gene expressibility.
• Associates with a chromatin-remodeling complex, suggesting a role in preventing expression of genes that trigger mitosis.
• Binds to monomethylated and dimethylated lysine 20 on histone H4 (H4K20me1/me2).
• Also recognizes histone H3 peptides monomethylated or dimethylated at lysine 4, lysine 9, or lysine 27 (H3K4me1/me2, H3K9me1/me2, H3K27me1/me2).
• Localizes to the nucleus, consistent with its function in chromatin regulation and gene silencing.
• Contains zinc-finger domains and undergoes alternative splicing; post-translational modifications include phosphorylation and ubiquitination-like conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 486–705 of human L3MBTL2 (NP_113676.2), a region within the C-terminal portion of the protein. This region may influence epitope accessibility; consider testing nuclear extracts or whole-cell lysates for detection.
• For Western blot (WB), the predicted molecular weight is approximately 79 kDa. Optimize gel percentage and transfer conditions based on this size. Confirm specificity by including positive (e.g., nuclear fractions) and negative controls.
• For ELISA, ensure the coating antigen is compatible with the polyclonal nature of the antibody. Validate performance using recombinant L3MBTL2 protein or peptide fragments.
• This antibody is reported to react with human L3MBTL2. Cross-reactivity with other species or isoforms has not been characterized; validate in the intended sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For chromatin biology and Polycomb group targets, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. We also support rare target raw-material sourcing to accelerate your assay development and research programs.