Anti-L2HGDH Polyclonal Antibody for WB, ELISA - Q9H9P8

Core Product Specifications and Parameters

Biological Background: L2HGDH Function and Localization

• L2HGDH (L-2-hydroxyglutarate dehydrogenase), also known as Duranin, is a mitochondrial enzyme encoded by the L2HGDH gene (C14orf160) in humans.
• It catalyzes the oxidation of L-2-hydroxyglutarate to 2-oxoglutarate, playing a key role in mitochondrial metabolism.
• This protein belongs to the FAD-dependent oxidoreductase family and requires FAD as a cofactor for its enzymatic activity.
• L2HGDH is localized to the mitochondrion, as indicated by its transit peptide and mitochondrial targeting sequence.
• Widely expressed across tissues, with highest levels in brain, testis, and muscle, suggesting critical roles in these metabolically active tissues.
• Alternative splicing produces multiple transcript variants, potentially influencing protein function or regulation.
• Post-translational modifications include acetylation, which may modulate enzyme activity or stability.
• Mutations in L2HGDH are associated with disease, underscoring its clinical relevance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-220), so the antibody is expected to recognize this portion of the protein. Consider this when designing epitope-tagged or truncated constructs.
• For WB, the predicted molecular weight is ~50 kDa; use an appropriate gel percentage and optimize transfer conditions for this size range. Validate detection in relevant lysates, such as brain or testis, where expression is high.
• For ELISA, a recombinant protein standard based on the immunogen sequence may be helpful for quantitative analysis.
• Shipment is on ice bags; upon arrival, aliquot and store at -20°C for long-term stability. Avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For L2HGDH-related research in mitochondrial disorders and metabolic diseases, we offer tailored sourcing solutions to accelerate your assay development from concept to clinic. Let CamelBio be your reliable partner in securing high-quality reagents for your next breakthrough.