Anti-L-Myc Rabbit Polyclonal Antibody for WB, ELISA - P12524

Core Product Specifications and Parameters

Biological Background: L-Myc Function and Localization

• L-Myc (MYCL, bHLHe38) is a member of the Myc family of basic helix-loop-helix (bHLH) transcription factors.
• It is encoded by the MYCL gene (also known as LMYC, MYCL1) and is classified as a DNA-binding protein involved in gene regulation.
• The protein localizes predominantly to the nucleus, consistent with its role in transcriptional control.
• Alternative splicing generates multiple isoforms, expanding its functional diversity.
• L-Myc shares homology with the viral oncogene v-myc, and it is implicated in cellular proliferation and oncogenesis.
• Structural and functional studies have been supported by direct protein sequencing and proteomics identification.
• The calculated molecular weight of the canonical isoform is approximately 40.3 kDa, matching the observed 40 kDa band in applications.
• UniProt keywords include Alternative splicing, DNA-binding, Nucleus, and Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 250–350, a region often involved in protein dimerization and DNA binding; verify antibody specificity if alternative splicing modifies this epitope in your sample system.
• For western blot, expect a band near 40 kDa under reducing conditions; optimize blocking conditions and antibody dilution using nuclear extracts or whole-cell lysates from human cell lines.
• In ELISA applications, consider using recombinant L-Myc protein or the immunizing peptide as a positive control; cross-reactivity with other Myc family members (c-Myc, N-Myc) may require independent validation.
• Prepare samples with nuclear extraction buffers to enrich for L-Myc if whole-cell lysates yield weak signals, reflecting its primary nuclear localization.
• Validate the antibody in the relevant human tissue or cell model before large-scale studies, as expression levels may vary among different cell types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, technical services, and consulting. For transcription factor studies targeting L-Myc and related oncoproteins, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets. Whether you need routine western blot antibodies or customized development support, CamelBio bridges your workflow from concept to clinic with reliable, one-stop supply solutions.