Anti-KYNU Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16719

Core Product Specifications and Parameters

Biological Background: Kynureninase Function and Localization

• Kynureninase (KYNU) is an enzyme encoded by the KYNU gene in humans; it is also known as L-kynurenine hydrolase.
• Catalyzes the cleavage of L-kynurenine (L-Kyn) into anthranilic acid (AA) and L-3-hydroxykynurenine (L-3OHKyn) into 3-hydroxyanthranilic acid (3-OHAA), with preference for the L-3-hydroxy form.
• Possesses cysteine-conjugate-beta-lyase activity, expanding its role in amino acid metabolism.
• Localizes predominantly to the cytoplasm and cytosol of cells.
• Ubiquitously expressed in human tissues, with highest levels in placenta, liver, and lung; detected in all brain regions.
• Key keywords: 3D-structure determined, subject to acetylation and alternative splicing, associated with disease variants, and dependent on pyridoxal phosphate as a cofactor.
• The protein has a calculated molecular weight of approximately 52 kDa, consistent with the supplier-provided weight.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal region (aa 1–307) of human KYNU. This region may be conserved across species, supporting the reported cross-reactivity with mouse and rat.
• For Western blot (WB), a band at approximately 52 kDa is expected; consider using placenta, liver, or lung lysates as positive controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), staining should be cytoplasmic; validate in tissues with known KYNU expression, such as placenta or brain regions.
• ELISA performance may be assessed using recombinant KYNU protein or capture-detection antibody pairs; include appropriate controls.
• When working with mouse or rat samples, verify specificity by sequence alignment and, if necessary, by knockdown or knockout validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive, one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. In the kynurenine pathway and tryptophan metabolism field, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare-target raw materials. Our supply capabilities help accelerate your assay development and ensure consistent reagent quality.