Anti-KYNU Monoclonal Antibody for WB, ELISA - Q16719

Core Product Specifications and Parameters

Biological Background: KYNU Function and Localization

• KYNU (Kynureninase) is an enzyme also referred to as KYNUU or L-kynurenine hydrolase, encoded by the KYNU gene.
• It catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic acid and 3-hydroxyanthranilic acid, respectively, playing a central role in tryptophan metabolism; it also has cysteine-conjugate-beta-lyase activity.
• Alternative splicing generates multiple isoforms; the canonical protein has a predicted molecular weight of ~52 kDa, while a 34 kDa isoform may also exist.
• Subcellular localization is cytoplasmic, primarily in the cytosol.
• KYNU is broadly expressed across human tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas, with highest levels in placenta, liver, and lung; expression is also detected in all brain regions.
• The enzyme requires pyridoxal phosphate as a cofactor and is involved in pyridine nucleotide biosynthesis.
• Post-translational modifications include acetylation; it is part of the human reference proteome and disease-associated variants have been documented.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full-length protein (aa 1-465), which may enable detection of multiple isoforms or cleavage products in Western blot.
• For Western blotting, consider using whole-cell or tissue lysates from human samples; the predicted molecular weights of 34 kDa and 52 kDa correspond to known isoforms.
• In ELISA applications, the antibody can be used in direct or capture formats; optimization of coating concentrations and incubation conditions is recommended.
• Given the enzyme's broad tissue distribution, validate expression in the relevant sample system (e.g., liver, placenta, or brain) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, delivering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target sourcing. This anti-KYNU rabbit monoclonal antibody facilitates research into tryptophan metabolism and related pathways. From concept to clinic, rely on CamelBio for consistent supply and expert technical guidance.