Anti-KYAT3 Rabbit Polyclonal Antibody (KAT3/CCBL2) for IHC-P, IF/ICC, ELISA - Q6YP21

Core Product Specifications and Parameters

Biological Background: KYAT3 Function and Localization

• Encodes kynurenine--oxoglutarate transaminase 3 (KYAT3), also known as kynurenine aminotransferase III (KATIII) and cysteine-S-conjugate beta-lyase 2 (CCBL2). Multiple isoforms are generated by alternative splicing.
• Catalyzes the irreversible transamination of L-kynurenine, an L-tryptophan metabolite, to form kynurenic acid (KA). KA acts as a broad-spectrum antagonist of ionotropic excitatory amino acid receptors, linking KYAT3 to neuromodulation and neuroprotection.
• Functions as a cysteine-S-conjugate beta-lyase, catalyzing the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, thereby cleaving C–S or C–Se bonds. This lyase activity suggests a role in the metabolism of xenobiotics and endogenous compounds.
• Exhibits broad substrate specificity in vitro, using glyoxylate as a preferred amino group acceptor. Active towards L-kynurenine, tryptophan, phenylalanine, serine, cysteine, methionine, histidine, glutamine, and asparagine. Lower activity is observed with 2-oxoglutarate.
• Belongs to the pyridoxal phosphate (PLP)-dependent aminotransferase family, consistent with its classification as a transferase. PLP serves as a critical cofactor for enzymatic activity.
• The protein undergoes post-translational acetylation, a modification that may regulate its activity, stability, or subcellular localization.
• Referenced in proteomics identification studies and designated as a reference proteome member, underscoring its fundamental role in human biology.

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide spanning amino acids 295–394 of human KYAT3 (isoform 1). This region lies within the catalytic domain near the PLP-binding site, which may enhance specificity for functional forms of the enzyme. For experiments targeting other isoforms, verify sequence alignment.
• This polyclonal antibody is qualified for IHC-P, IF/ICC, and ELISA. For immunohistochemistry, consider antigen retrieval methods compatible with the tissue type to maximize signal. Using positive control tissues known to express KYAT3 (e.g., liver, kidney) is recommended.
• Given its polyclonal nature, lot-to-lot consistency should be monitored. Perform routine validation in the relevant sample system and include appropriate negative controls (e.g., blocking peptide pre-absorption) to confirm specificity.
• For ELISA applications, the antibody can be used for either direct or capture ELISA formats. Optimize coating concentration and detection label conditions based on the assay design.
• Cross-reactivity with mouse KYAT3 has been confirmed, but sequence homology should be examined if used in other species. A sequence alignment of the immunogen region is advisable when working with non-validated species.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated partner for diagnostic manufacturers, laboratories, and research institutes, CamelBio streamlines the procurement of IVD raw materials from concept to clinic. This anti-KYAT3 antibody targets a key enzyme in tryptophan metabolism—valuable for developing assays in neuroscience and metabolic disorders. Explore our extensive portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets. Contact us to discuss your specific project needs.