Anti-Ku80 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P13010

Core Product Specifications and Parameters

Biological Background: Ku80 Function and Localization

• Encoded by XRCC5, Ku80 (also known as Ku86, NFIV, and lupus autoantigen p86) localizes to the nucleus, nucleolus, and chromosomes, forming a stable heterodimer with Ku70 (XRCC6).
• The Ku complex is the primary sensor for DNA double-strand breaks (DSBs), initiating canonical non-homologous end joining (NHEJ) and V(D)J recombination. This pathway ensures rapid repair of cytotoxic DSBs and contributes to immune diversity. Related references: PMID:11493912, PMID:33854234, PMID:34352203, PMID:9742108
• Likely possesses 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase) activity, catalyzing beta-elimination at abasic sites near DSBs to clean broken ends prior to ligation. Related references: PMID:20383123
• May also have 3'-5' DNA helicase activity, though its physiological relevance in vivo remains unconfirmed. Related references: PMID:7957065
• Plays a role in telomere maintenance, contributing to genomic stability. Related references: PMID:29490055
• Acts as a transcriptional cofactor for various transcription factors, thereby regulating gene expression. Related references: PMID:12145306, PMID:8621488
• Involved in innate immunity by assembling into the HDP-RNP complex, which serves as a platform for IRF3 phosphorylation and activation of the cGAS-STING pathway. Related references: PMID:28712728
• Binds RNAs and recruits PRKDC to a wide range of cellular RNAs, including U3 small nucleolar RNA, thereby playing a role in ribosomal RNA biogenesis. Related references: PMID:32103174

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (amino acids 543–732) of human Ku80, a domain conserved across species; this may account for the observed cross-reactivity with mouse and rat.
• For Western blotting, the predicted molecular weight is 83kDa; consider validating the band in nuclear-enriched lysates from human, mouse, or rat cell lines.
• The antibody is reactive in multiple applications (WB, IHC-P, IF/ICC, IP, ELISA); optimize dilutions and incubation conditions for each assay.
• In immunofluorescence, Ku80’s nuclear and nucleolar localization suggests that permeabilization with detergents (e.g., Triton X-100) may be necessary to achieve optimal staining.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for IVD raw materials, providing one-stop access to validated antibodies like this anti-Ku80 polyclonal antibody. We support DNA repair and immunoassay research with optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. From concept to clinic, CamelBio streamlines your diagnostic development with reliable raw-material supply and expert technical consultation.