Anti-Ku80 Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P27641

Core Product Specifications and Parameters

Biological Background: Ku80 Function and Localization

• Ku80 (also known as XRCC5, Ku86, CTC85, CTCBF) is a DNA repair protein that forms a heterodimer with Ku70 (XRCC6) to create the Ku complex, which serves as a regulatory subunit of the DNA-dependent protein kinase (DNA-PK).
• The Ku complex binds to DNA double-strand breaks (DSBs) and is essential for the non-homologous end joining (NHEJ) pathway, which repairs cytotoxic and mutagenic DSBs and is required for V(D)J recombination to generate antibody and T-cell receptor diversity.
• Ku80 likely possesses 5'-deoxyribose-5-phosphate (5'-dRP) lyase activity, catalyzing the removal of abasic sites near DSBs to prepare broken ends for ligation.
• Beyond DNA repair, Ku80 contributes to telomere maintenance and acts as a transcriptional regulator by serving as a cofactor for various transcription factors.
• Ku80 plays a role in innate immunity by assembling into the HDP-RNP complex, which facilitates IRF3 phosphorylation and activates the cGAS-STING pathway in response to DNA viruses.
• It can also bind RNA and recruits PRKDC to cellular RNAs, including the U3 small nucleolar RNA, implicating it in ribosomal RNA biogenesis.
• Subcellularly, Ku80 localizes to the nucleus, nucleolus, and chromosomes, consistent with its roles in DNA repair, transcription, and ribosome biogenesis.
• Post-translational modifications include acetylation, phosphorylation, ADP-ribosylation, and ubiquitination, which may regulate its multiple functions.

Experimental Guidance and Technical Tips

• The immunogen was designed based on a fragment of mouse Ku80 (aa 543-732); for experiments requiring full-length protein recognition, consider validating with the relevant sample system.
• This antibody is suitable for WB, IF/ICC, IHC-P, and ELISA; optimal dilutions should be experimentally determined for each application.
• Given the high homology between human and mouse Ku80, cross-reactivity is expected, but confirm in your specific experimental setup.
• For nuclear targets like Ku80, appropriate nuclear extraction or permeabilization protocols may be necessary for IF/ICC and IHC.

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