Anti-Ku80 Monoclonal Antibody for ELISA, IF/ICC, IHC-P, IP, WB - P13010

Core Product Specifications and Parameters

Biological Background: DNA Repair Protein Ku80 Function and Localization

• Ku80, also known as XRCC5, is the 86 kDa subunit of the Ku antigen. It heterodimerizes with XRCC6 (Ku70) to form the Ku complex, a DNA end‑binding platform essential for non‑homologous end joining (NHEJ)‑mediated repair of double‑strand breaks (PubMed:11493912, PubMed:33854234, PubMed:34352203) and for V(D)J recombination during T‑cell receptor and antibody diversification (PubMed:9742108). Related references: PMID:11493912, PMID:33854234, PMID:34352203, PMID:9742108

• Ku80 possesses 5′-deoxyribose‑5‑phosphate lyase activity, which cleans abasic site termini near DNA breaks to prepare ends for ligation (PubMed:20383123). Related references: PMID:20383123

• Ku80 may exhibit 3′‑5′ DNA helicase activity, although this has not been confirmed in vivo and its physiological relevance remains unclear (PubMed:7957065). Related references: PMID:7957065

• Ku80 contributes to telomere maintenance and telomere‑end protection (PubMed:29490055). Related references: PMID:29490055

• Ku80 acts as a transcriptional coregulator, functioning as a cofactor for various transcription factors (PubMed:12145306, PubMed:8621488). Related references: PMID:12145306, PMID:8621488

• Ku80 participates in the innate immune response to DNA viruses by assembling the HDP‑RNP complex, which activates IRF3 via the cGAS‑STING pathway (PubMed:28712728). Related references: PMID:28712728

• Ku80 binds various cellular RNAs and recruits the catalytic subunit of DNA‑PK (PRKDC), playing a role in ribosomal RNA biogenesis (PubMed:32103174). Related references: PMID:32103174

• Ku80 localizes primarily to the nucleus and nucleolus and associates with chromosomes. It undergoes several post‑translational modifications including acetylation, phosphorylation, ADP‑ribosylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 543–732 of human Ku80; therefore, this antibody may specifically recognize an epitope within the C‑terminal domain.
• For Western blotting, a band of approximately 83 kDa is expected; consider using whole‑cell lysates from human cell lines that express Ku80.
• For immunoprecipitation (IP), validate the antibody in your specific lysate and buffer conditions.
• For immunofluorescence (IF/ICC) and immunohistochemistry (IHC‑P), optimize fixation and antigen retrieval protocols, as the antibody may require epitope unmasking.
• For ELISA applications, pair‑testing with a detection antibody may be necessary; conduct appropriate titrations.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, CROs, and research laboratories with a single‑point access to a wide range of IVD raw materials, including validated antibody pairs, bulk‑grade monoclonals, and ancillary reagents. With expertise spanning DNA repair, NHEJ, and immune response pathways, we support your development projects from concept to clinic. Contact us to explore custom sourcing solutions for rare‑target proteins and related assay components.