Anti-Ku70 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P12956

Core Product Specifications and Parameters

Biological Background: Ku70 Function and Localization

• Ku70 (XRCC6) is a key DNA-binding protein that mediates DNA double-strand break (DSB) repair through the classical non-homologous end joining (NHEJ) pathway. It forms a heterodimer with Ku80 (XRCC5) to create the Ku complex, which specifically recognizes and binds broken DNA ends, serving as a scaffold to recruit essential repair factors. (PubMed:11493912, PubMed:20493174, PubMed:33854234) Related references: PMID:11493912, PMID:20493174, PMID:33854234
• The Ku-dependent NHEJ pathway is critical for V(D)J recombination, the process that generates antibody and T-cell receptor diversity, highlighting its essential role in adaptive immunity. (PubMed:9742108) Related references: PMID:9742108
• Ku70 possesses 5'-deoxyribose-5-phosphate (5'-dRP) lyase activity, which cleans abasic sites near DSBs to prepare DNA termini for ligation. In vitro studies also suggest a 3'-5' DNA helicase activity, though its physiological relevance remains unclear. (PubMed:20383123, PubMed:7957065) Related references: PMID:20383123, PMID:7957065
• Beyond repair, Ku70 contributes to telomere maintenance and acts as a transcriptional cofactor for various transcription factors, linking DNA repair to broader cellular processes. (PubMed:29490055, PubMed:12145306, PubMed:8621488) Related references: PMID:29490055, PMID:12145306, PMID:8621488
• Ku70 plays a role in innate antiviral immunity by assembling the HDP-RNP complex, which facilitates IRF3 phosphorylation and activation of the cGAS-STING pathway. (PubMed:28712728) Related references: PMID:28712728
• It can also bind cellular RNAs and recruit PRKDC to participate in ribosomal RNA biogenesis, indicating functional versatility. (PubMed:32103174) Related references: PMID:32103174
• Ku70 negatively regulates apoptosis by interacting with and sequestering BAX away from mitochondria; it may also deubiquitinate BAX. (PubMed:15023334, PubMed:18362350, PubMed:35545041) Related references: PMID:15023334, PMID:18362350, PMID:35545041
• Subcellularly, Ku70 is primarily nuclear and chromosome-associated but can also be found in the cytoplasm under certain conditions, consistent with its multiple functions.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to amino acids 501-600 of human Ku70. This internal region is expected to generate antibodies recognizing the native, full-length protein. Consider validating antibody specificity using knockdown or knockout cell lysates.
• Given the listed applications (WB, IHC-P, IF/ICC, IP, ELISA), it is advisable to optimize dilutions experimentally for each assay. For Western blot, a band around 70 kDa is expected; using reducing conditions is typical for this target.
• Cross-reactivity with mouse and rat Ku70 is reported; users should confirm performance in their specific sample systems. For IHC and IF, appropriate fixation and permeabilization protocols should be tested.
• As polyclonal antibodies may exhibit lot-to-lot variation, include appropriate positive and negative controls in all experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive B2B partner for IVD raw-material procurement, providing one-stop access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and support for rare target sourcing. With this anti-Ku70 polyclonal antibody, researchers investigating DNA repair, NHEJ pathways, or related disease models can efficiently integrate high-quality immunoreagents into their workflow. Contact us to explore customized sourcing solutions that streamline your development from concept to clinic.