Anti-Ku70 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P12956

Core Product Specifications and Parameters

Biological Background: Ku70 Function and Localization

• Essential DNA-binding protein that forms the Ku70:Ku80 heterodimer (Ku complex), acting as a primary sensor and binding platform for DNA double-strand breaks (DSBs) and initiating the classical non-homologous end joining (NHEJ) repair pathway. It recruits core NHEJ factors, including PRKDC, to align and ligate broken DNA ends, ensuring genomic stability and enabling V(D)J recombination in immune cells. Related references: PMID:11493912 | PMID:20493174 | PMID:33854234 | PMID:9742108

• Possesses 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase) activity, which cleans abasic sites at DSB termini via beta-elimination, preparing broken ends for direct ligation during NHEJ. Related references: PMID:20383123

• Functions as a transcriptional co-regulator by interacting with various transcription factors, thereby influencing gene expression programs beyond its canonical DNA repair role. Related references: PMID:12145306 | PMID:8621488

• Participates in innate immune responses to DNA viruses by assembling into the HDP-RNP complex, which serves as a platform for IRF3 phosphorylation and activation of the cGAS-STING pathway. Related references: PMID:28712728

• Binds both DNA and RNA; interacts with cellular RNAs, including U3 snoRNA, and recruits PRKDC to facilitate ribosomal RNA biogenesis. Related references: PMID:32103174

• Negatively regulates apoptosis by sequestering the pro-apoptotic protein BAX away from mitochondria and may deubiquitinate BAX, providing a cytoprotective mechanism. Related references: PMID:15023334 | PMID:18362350 | PMID:35545041

• Primarily localized in the nucleus and associated with chromosomes, but also detected in the cytoplasm, consistent with its multifunctional roles in DNA repair, telomere maintenance, and apoptosis regulation. Alternative splicing yields multiple isoforms, and the protein undergoes various post-translational modifications, including acetylation, phosphorylation, and ADP-ribosylation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 408-609 of human Ku70 (NP_001460.1). This region lies in the C-terminal portion of the protein. Consider verifying specificity in your system, as polyclonal antibodies may recognize multiple isoforms or degradation products.
• Antibody performance has been reported by the supplier for WB, IHC-P, IF/ICC, and ELISA. For each application, optimize dilutions and detection conditions using appropriate positive (e.g., HeLa, HEK293) and negative controls.
• Cross-reactivity is expected with mouse and rat Ku70 based on sequence homology, but signal intensity or background may vary among species; validate in the relevant sample system.
• As a rabbit polyclonal antibody, lot-to-lot consistency can differ from monoclonal preparations. Include internal reference standards and document lot numbers for reproducibility.
• For IHC-P, antigen retrieval conditions and detection methods should be empirically determined. In IF/ICC, confirm nuclear and cytoplasmic localization patterns consistent with Ku70’s described subcellular distribution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This Ku70 antibody targets a key player in DNA damage response pathways, suitable for assay development and research applications. Through our integrated sourcing and technical support, we help accelerate your projects from concept to clinic with reliable, high-quality raw materials and expert consulting.