Anti-KTN1 Polyclonal Antibody for WB and ELISA - Q86UP2

Core Product Specifications and Parameters

Biological Background: KTN1 Function and Localization

• KTN1 encodes the protein kinectin, also known as CG-1 antigen or kinesin receptor, which acts as a receptor for kinesin, facilitating kinesin-driven vesicle motility along microtubules.
• Upon fibronectin-induced integrin aggregation, kinectin accumulates in integrin-based adhesion complexes (IAC), linking vesicle transport to cell adhesion.
• Kinectin is a single-pass type I membrane protein anchored in the endoplasmic reticulum (ER) membrane, with its functional N-terminus facing the cytoplasm.
• The protein undergoes alternative splicing and contains coiled-coil regions, suggesting roles in protein–protein interactions.
• Post-translational modifications include phosphorylation and glycosylation, which may regulate its activity and localization.
• Tissue expression is highest in peripheral blood lymphocytes, testis, and ovary, with lower levels in spleen, thymus, prostate, small intestine, and colon.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1–100) of human KTN1, which is conserved across human, mouse, and rat, supporting the observed cross-reactivity. When performing Western blot, consider using lysates from testis or ovary, where expression is high, to detect the ~156 kDa band.
• For ELISA applications, the antibody may be used as a capture or detection reagent; optimize working concentrations with your specific antigen and assay format.
• Given the ER membrane localization, subcellular fractionation or enrichment steps may improve detection sensitivity in low-expressing samples.
• As a polyclonal antibody, lot-to-lot consistency should be verified, and working dilutions should be titrated for each new batch.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering a wide range of validated antibodies, recombinant proteins, and ancillary reagents. This anti-KTN1 polyclonal antibody supports research into kinesin-driven transport and ER dynamics. Beyond catalog products, we can assist with customized antibody generation, bulk supply, and procurement of rare targets to help you move from early development to clinical application seamlessly.